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Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants

dc.contributor.authorOnyilo, F.
dc.contributor.authorTusiime, Geoffrey
dc.contributor.authorTripathi, J.N.
dc.contributor.authorChen, L.H.
dc.contributor.authorFalk, B.
dc.contributor.authorStergiopoulos, I.
dc.contributor.authorTushemereirwe, W.K.
dc.contributor.authorKubiriba, J.
dc.contributor.authorTripathi, L.
dc.date.accessioned2019-12-04T11:18:26Z
dc.date.available2019-12-04T11:18:26Z
dc.date.issued2018-03-13
dc.identifier.citationOnyilo, F., Tusiime, G., Tripathi, J.N., Chen, L.H., Falk, B., Stergiopoulos, I., ... & Tripathi, L. (2018). Silencing of the mitogen activated protein kinases (MAPK) fus3 and slt2 in Pseudocercospora fijiensis reduces growth and virulence on host plants. Frontiers in Plant Science, 9(291), 1-12.
dc.identifier.issn1664-462X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3203
dc.descriptionOpen Access Journal
dc.description.abstractPseudocercospora fijiensis, causal agent of the black Sigatoka disease (BSD) of Musa spp., has spread globally since its discovery in Fiji 1963 to all the banana and plantain growing areas across the globe. It is becoming the most damaging and economically important disease of this crop. The identification and characterization of genes that regulate infection processes and pathogenicity in P. fijiensis will provide important knowledge for the development of disease-resistant cultivars. In many fungal plant pathogens, the Fus3 and Slt2 are reported to be essential for pathogenicity. Fus3 regulates filamentous-invasion pathways including the formation of infection structures, sporulation, virulence, and invasive and filamentous growth, whereas Slt2 is involved in the cell-wall integrity pathway, virulence, invasive growth, and colonization in host tissues. Here, we used RNAi-mediated gene silencing to investigate the role of the Slt2 and Fus3 homologs in P. fijiensis in pathogen invasiveness, growth and pathogenicity. The PfSlt2 and PfFus3 silenced P. fijiensis transformants showed significantly lower gene expression and reduced virulence, invasive growth, and lower biomass in infected leaf tissues of East African Highland Banana (EAHB). This study suggests that Slt2 and Fus3 MAPK signaling pathways play important roles in plant infection and pathogenic growth of fungal pathogens. The silencing of these vital fungal genes through host-induced gene silencing (HIG) could be an alternative strategy for developing transgenic banana and plantain resistant to BSD.
dc.description.sponsorshipUnited States Agency for International Development
dc.format.extent1-12
dc.language.isoen
dc.subjectPseudocercospora
dc.subjectProtein Kinase
dc.subjectPathogenicity
dc.subjectBlack Sigatoka Disease
dc.subjectPlantains
dc.subjectBananas
dc.subjectHost Plant
dc.titleSilencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMakerere University
cg.contributor.affiliationNational Agricultural Research Organisation, Uganda
cg.contributor.affiliationUniversity of California
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.creator.identifierLeena Tripathi: 0000-0001-5723-4981
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectBanana
cg.iitasubjectPlant Production
cg.iitasubjectPlantain
cg.journalFrontiers in Plant Science
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid94835
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.3389/fpls.2018.00291


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