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Morphological diversity and genomic DNA fingerprinting of the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) and of two other species of African Orseolia

dc.contributor.authorNwilene, F.E.
dc.contributor.authorHarris, K.M.
dc.contributor.authorOkhidievbie, O.
dc.contributor.authorOnasanya, A.
dc.contributor.authorSere, Y.
dc.contributor.authorIngelbrecht, I.L.
dc.date.accessioned2019-12-04T11:18:35Z
dc.date.available2019-12-04T11:18:35Z
dc.date.issued2006
dc.identifier.citationNwilene, F.E., Harris, K.M., Okhidievbie, O., Onasanya, A., Sere, Y. & Ingelbrecht, I. (2006). Morphological diversity and genomic DNA fingerprinting of the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) and of two other species of African Orseolia. International Journal of Tropical Insect Science, 26(4), 256-265.
dc.identifier.issn1742-7584
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3259
dc.description.abstractA morphological diversity study was conducted on African rice gall midge Orseolia oryzivora Harris & Gagné and two other African species, Orseolia bonzii Harris and Orseolia nwanzei Harris & Nwilene, sp. n. Morphological differences between adult and immature stages of the three species are slight and mainly evident in pupal characters. Due to limited differences in morphological characters, genomic DNA fingerprinting of these three insect species was carried out using random amplified polymorphic DNA (RAPD) and sequence-characterized amplified region (SCAR) methods. Out of 90 operon primers, 15 showed polymorphism among the three species tested, generating 86 bands, 60 (70%) of which were polymorphic. Primer OPV6 produced three RAPD markers that distinguished the three Orseolia species. These markers were cloned and sequenced. Their sequence was then used to design six SCAR primer pairs. Each SCAR primer pair amplified and distinguished the three Orseolia species at genomic DNA level. Both SCAR and RAPD genomic DNA fingerprinting revealed that O. oryzivora and O. bonzii are most closely related and O. nwanzei is distinct. Each of the six SCAR primer pairs produced a specific DNA fragment size specific for O. nwanzei, O. oryzivora and O. bonzii. The DNA fingerprints will be useful for entomological survey, identification of new species and differentiating aggressive from non-aggressive species, aimed at the effective development of rice cultivars with durable resistance to African rice gall midge.
dc.description.abstractUne etude de la djversite morphologique a ete reaHsee sur la cecidomyie de la galle du riz africain, Orseolia oryzivora Harris & Gagne et sur deux autres especes africaines, O. bonzii Harris et O. nwallzei Harris & Nwilene, sp. n. Les difference morphologiques entre les stades adultes et immatures des trois especes sont minimes et plus marquees au niveau des caracteres nymphaux. A cause des caracteres morphologiques limites, la cartographie peptidique de l' ADN genomique de ces trois especes d'insectes a ete effectuee a I'aide de la methode d'amplification aleatoire d'ADN polymorphe (RAPD) et de la methode SCAR. Quinze des 90 amorces d'operons ont montre un polymorphisme chez les trois especes testees, creant 86 bandes, dont 60 (70%) etaient polymorphes. L'amorce OPV6 a produit trois marqueurs RAPD, qui ont permis de differencier les trois especes d'Orseolia. Ces marqueurs ont ete clones et sequences. Leur sequence a ete ensuite utilisee pour concevoir six paires d'amorces SCAR. Les paires des amorces de chaque marqueur SCAR ont permis d'amplifier et de differencier les trois especes d'Orseolin au niveau de l' ADN genomique. La cartographie peptidique de I' ADN genomique des marqueurs SCAR et RAPD a revele que les especes O. oryzivorn et O. bOllzii sont plus etroitement liees et que O. Ilwallzei est distincte. Chacune des six paires d'amorces SCAR a produit un segment d' ADN de taille specifique pour O. muanzei, o oryzivorn et 0, bOllzii; ces paires d'amorces pourraient donc etre utiles pour des diagnostiques de terrain. La cartographie peptidique de l' ADN de£inie pour chaque espece d'Orseo/ia sera utile pour les etudes entomologiques, !'identification de nouvelles especes et la differentiation des especes agressives des especes non agressives, en vue de developper de fa,on efficace des cultivars de riz dotes d'une resistance durable a la cecidomyie de la galle du riz africain.
dc.language.isoen
dc.language.isofr
dc.subjectAfrican Rice Gall Midge
dc.subjectOrseolin Oryzivom
dc.subjectOrseolin Boilzii
dc.subjectOrseolia Nwnnzei
dc.subjectDna
dc.subjectFingerprinting
dc.subjectScar
dc.subjectRice
dc.subjectEmgrostis Atroviren
dc.subjectCecidomyie De La Galle Du Riz Africain
dc.subjectOrseolin Oryzivorn
dc.subjectOrseolin Bonzii
dc.subjectOrseolin Ilwnllzei
dc.subjectAdn
dc.subjectCartographie Peptidique
dc.subjectScar
dc.subjectRiz
dc.subjectEragrostis Alrovirells
dc.titleMorphological diversity and genomic DNA fingerprinting of the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) and of two other species of African Orseolia
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationAfrica Rice Center
cg.contributor.affiliationLinden Way, United Kingdom
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Breeding
cg.accessibilitystatusLimited Access
local.dspaceid94933
cg.identifier.doihttp://dx.doi.org/10.1017/S1742758406694058


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