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Detection of RAPD markers linked to resistance to cassava anthracnose disease

dc.contributor.authorAkinbo, Olalekan
dc.contributor.authorGedil, M.
dc.contributor.authorEkpo, E.J.A.
dc.contributor.authorOladele, J.
dc.contributor.authorDixon, Alfred G.O.
dc.date.accessioned2019-12-04T11:19:22Z
dc.date.available2019-12-04T11:19:22Z
dc.date.issued2007-03
dc.identifier.citationAkinbo, O., Gedil, M., Ekpo, E.J.A., Oladele, J. & Dixon, A.G. (2007). Detection of RAPD markers-linked to resistance to cassava anthracnose disease. African Journal of Biotechnology, 6(6), 677-682.
dc.identifier.issn1684-5315
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3453
dc.description.abstractCassava anthracnose disease (CAD) is a major stem disease of cassava (Manihot esculenta Crantz) in the cassava growing areas of the world. To identify markers associated with resistance to CAD, F1 progenies were produced from a cross between resistant genotype TME 117, and susceptible genotype TMS 920326, as the experimental population. The chi-square test gave a goodness of fit for the expected ratio of 1:1 for resistant to susceptible genotypes suggesting a monogenic dominant inheritance. Two hundred decamer primers were screened using both resistance and susceptible parent to CAD. Bulk segregant analysis was quickly used to search for Random amplified polymorphic DNA (RAPDs) linked to anthracnose resistance in F1 derived from TMS 920326 and TME 117. The fragment linked to the gene was flanked on both sides by primers OPAF2 and OPF06 at 13.1 and 22.2cM. To our knowledge this is the first report on this fungal disease of cassava and of molecular markers that is tag CAD resistance in cassava. We discuss the use of markers-linked to CAD1 for marker-assisted cassava breeding.
dc.language.isoen
dc.subjectBulk Segregant Analysis
dc.subjectCassava Anthracnose Disease
dc.subjectMolecular Marker-Assisted Selection
dc.subjectRapd
dc.subjectHost Resistance
dc.titleDetection of RAPD markers linked to resistance to cassava anthracnose disease
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationUniversity of Ibadan
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationGeorgetown University
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.regionNorth America
cg.coverage.countryNigeria
cg.coverage.countryUnited States
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Diseases
cg.iitasubjectDisease Control
cg.iitasubjectCassava
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Production
cg.iitasubjectLivelihoods
cg.iitasubjectGenetic Improvement
cg.accessibilitystatusOpen Access
local.dspaceid95424


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