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    Rapid screening of Musa species for resistance to black leaf streak using in vitro plantlets in tubes and detached leaves

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    Twizyimana, Banana black leaf streak early screening paper, plant disease, 2007.pdf (306.5Kb)
    Date
    2007
    Author
    Twizeyimana, M.
    Ojiambo, P.S.
    Tenkouano, A.
    Ikotun, T.
    Bandyopadhyay, Ranajit
    Type
    Journal Article
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    Abstract/Description
    This study investigated the utility of inoculation of in vitro plantlets in tubes and detached leaves as reliable and rapid assays for screening Musa genotypes against Mycosphaerella fijiensis, the causal agent of black leaf streak. In the first part of the study, three types of inocula were evaluated to determine suitability for in vitro inoculation. Inoculation of in vitro plantlets with mycelial fragments resulted in significantly (P< 0.05) higher levels of disease severity and faster rates of disease progress compared with inoculations using conidial suspensions. In the detached leaf assay, amending agar medium with plant hormones significantly (P< 0.0001) aided retention of green leaf color. Leaf pieces on medium containing gibberellic acid at 5mgliter had about 5% chlorosis at 52 days after plating. When in vitro plantlets in tubes and detached leaves of 10 Musa genotypes with different levels of disease resistance were inoculated with M. fijiensis, there were significant (P< 0.05) differences among genotypes in leaf area infected, incubation time, and symptom evolution time. For incubation time and leaf area infected, cultivars responded depending on their level of disease resistance, with resistant genotypes Calcutta-4 and PITA-17 having significantly (P= 0.001) longer incubation times and lower infected leaf areas compared with the susceptible cultivar Agbagba and moderately resistant cultivar FHIA-23. A similar pattern in cultivar response was observed for symptom evolution time. Leaf area infected was not significantly (P= 0.2817 for two-tailed t test) different when assessed using the two assays, and infected leaf areas in both assays were strongly correlated (r= 0.88, n= 48, P<0.0001). Although incubation times were significantly (P= 0.0062 for two-tailed ttest) different between the two assays, values from the two assays were strongly correlated (r= 0.69, n= 48, P< 0.0001). These results show that these two assays are rapid and space-effective, and can reliably be used for screening Musa genotypes for resistance to black leaf streak.
    https://doi.org/10.1094/PDIS-91-3-0308
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3466
    Digital Object Identifier (DOI)
    https://doi.org/10.1094/PDIS-91-3-0308
    IITA Subjects
    Banana; Food Security; Handling, Transport, Storage And Protection Of Agricultural Products; Plant Breeding; Plant Production; Agribusiness; Livelihoods; Disease Control; Genetic Improvement; Pests Of Plants; Plant Genetic Resources; Plant Diseases
    Agrovoc Terms
    Bananas; Black Sigatoka; Plantains; Inoculation; Musa Genotypes; Incubation; Disease Resistance; Cultivar; Black Leaf Streak
    Regions
    Africa; West Africa; Central Africa
    Countries
    Nigeria; Cameroon
    Collections
    • Journal and Journal Articles4835
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