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Comparison of field, greenhouse and detachedleaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi

dc.contributor.authorTwizeyimana, M.
dc.contributor.authorOjiambo, P.S.
dc.contributor.authorIkotun, T.
dc.contributor.authorPaul, C.
dc.contributor.authorHartman, G.L.
dc.contributor.authorBandyopadhyay, Ranajit
dc.date.accessioned2019-12-04T11:19:28Z
dc.date.available2019-12-04T11:19:28Z
dc.date.issued2007
dc.identifier.citationTwizeyimana, M., Ojiambo, P.S., Ikotun, T., Paul, C., Hartman, G.L. & Bandyopadhyay, R. (2007). Comparison of field, greenhouse, and detached-leaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi. Plant Disease, 91(9), 1161-1169.
dc.identifier.issn0191-2917
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3473
dc.description.abstractFourteen soybean accessions and breeding lines were evaluated for resistance to soybean rust caused by the fungus Phakopsora pachyrhizi. Evaluations were conducted in replicated experiments in growth chambers using detached leaves and under greenhouse and field conditions. In growth-chamber experiments, inoculation of detached leaves with 1 × 106 sporesml resulted in a significantly (P < 0.0001) higher total number of pustules and spores per unit leaf area than inoculations with lower spore concentrations. Amending agar medium with plant hormones significantly (P < 0.0001) aided retention of green leaf color in detached leaves. Leaf pieces on a medium containing kinetin at 10 mgliter had 5% chlorosis at 18 days after plating compared with leaf pieces on media amended with all other plant hormones, which had higher levels of chlorosis. Leaf age significantly affected number of pustules (P = 0.0146) and number of spores per pustule (P = 0.0088), and 3- to 4-week-old leaves had a higher number of pustules and number of spores per pustule compared with leaves that were either 1 to 2 or 5 to 6 weeks old. In detached-leaf and greenhouse screening, plants were evaluated for days to lesion appearance, days to pustule formation, days to pustule eruption, lesion number, lesion diameter, lesion type, number of pustules, and spores per pustule in 1-cm2 leaf area. Plants also were evaluated for diseased leaf area (in greenhouse and field screening) and sporulation (in field screening) at growth stage R6. There were significant (P < 0.0001) differences among genotypes in their response to P. pachyrhizi infection in the detached-leaf, greenhouse, and field evaluations. Accessions PI 594538A, PI 417089A, and UG-5 had very low levels of disease compared with the susceptible checks and all other genotypes. Detached-leaf, greenhouse, and field results were comparable, and there were significant correlations between detached-leaf and greenhouse (absolute r = 0.79; P < 0.0001) and between detached-leaf and field resistance (absolute r = 0.83; P < 0.0001) across genotypes. The overall results show the utility of detached-leaf assay for screening soybean for rust resistance.
dc.language.isoen
dc.subjectDisease Resistance
dc.subjectSoybeans
dc.subjectPlant Hormones
dc.subjectChlorosis
dc.subjectSpores Per Pustule
dc.subjectGenotypes
dc.subjectPhakopsora Pachyrhizi
dc.subjectDetached-Leaf
dc.titleComparison of field, greenhouse and detachedleaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Ibadan
cg.contributor.affiliationUniversity of Illinois
cg.contributor.affiliationNational Soybean Research Center
cg.coverage.regionAfrica
cg.coverage.regionAcp
cg.coverage.regionWest Africa
cg.coverage.regionNorth America
cg.coverage.countryNigeria
cg.coverage.countryUnited States
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectNutrition
cg.iitasubjectSoybean
cg.iitasubjectFood Security
cg.iitasubjectDiseases Control
cg.iitasubjectGenetic Improvement
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Diseases
cg.iitasubjectLivelihoods
cg.iitasubjectPlant Production
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.accessibilitystatusLimited Access
local.dspaceid95444
cg.identifier.doihttps://doi.org/10.1094/PDIS-91-9-1161


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