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First report of rust caused by Phakopsora pachyrhizi on soybean in Democratic Republic of Congo

dc.contributor.authorOjiambo, P.
dc.contributor.authorBandyopadhyay, Ranajit
dc.contributor.authorTwizeyimana, M.
dc.contributor.authorLema, A.
dc.contributor.authorFrederick, R.D.
dc.contributor.authorPedley, K.F.
dc.contributor.authorStone, C.L.
dc.contributor.authorHartman, G.
dc.date.accessioned2019-12-04T11:19:29Z
dc.date.available2019-12-04T11:19:29Z
dc.date.issued2007
dc.identifier.citationOjiambo, P.S., Bandyopadhyay, R., Twizeyimana, M., Lema, A., Frederick, R.D., Pedley, K.F., ... & Hartman, G.L. (2007). First report of rust caused by Phakopsora pachyrhizi on soybean in Democratic Republic of Congo. Plant Disease, 91(9), 1204-1205.
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3474
dc.description.abstractNigeria (1) and Uganda (3) are the closest countries to the Democratic Republic of Congo (DRC) where soybean rust caused by Phakopsora pachyrhizi has been reported. In February 2007, during a disease survey in DRC, soybean (Glycine max) leaves with rust symptoms (tan, angular lesions with erumpent sori exuding urediniospores) were observed in 10 fields in the following areas in Bas Congo Province: Bangu, Kimpese, Kolo-Fuma, Lukala, Mbanza-Ngungu, Mpalukide, Mvuazi, and Ntemo. Rust incidence in these fields ranged from 85 to 100%, while severity ranged between 3 and 35% of the leaf area on infected plants. Urediniospores were hyaline, minutely echinulate, and 23 to 31 × 16 to 20 μm. Within a week of collection, infected leaf samples were sent to the USDA-ARS Foreign Disease-Weed Science Research Unit (FDWSRU) for pathogen identification. DNA was extracted from sections of leaves containing sori with the Qiagen DNeasy Plant Mini kit (Valencia, CA), and all 10 field samples amplified in a real-time fluorescent PCR with the P. pachyrhizi-specific primers Ppm1 and Ppa2 (2). Infected leaves of cultivar Vuangi collected from one field each in the INERA Research Station, Kimpese-Crawford, and Kimpese-Ceco were separately washed in sterile water to collect urediniospores that were used to separately inoculate three detached leaves of susceptible cultivar TGx 1485-1D (4). Lesions on inoculated leaves developed 5 days after inoculation (DAI), and pustules (110 to 130 μm) formed 7 DAI and erupted 2 days later exuding columns of urediniospores similar in size to the initially collected isolates. Inoculation of another set of detached leaves with a spore suspension (1 × 106 spores per ml) from the first set of detached leaves resulted in typical rust symptoms. Seedlings of cultivar Williams also showed typical rust symptoms when inoculated separately with urediniospores collected from nine fields (i.e., all except Kimpese-Ceco, which was infective in the detached leaf assay). Inoculation and incubation were carried out at the FDWSRU Plant Pathogen Containment Facility at Fort Detrick as described earlier (2). The PCR assay, morphological characters of the isolates, and pathogenicity tests demonstrate that P. pachyrhizi occurs in DRC. To our knowledge, this is the first report of P. achyrhiziinfecting soybean in DRC.
dc.language.isoen
dc.subjectPhakopsora Pachyrhizi
dc.subjectSoybeans
dc.subjectUrediniospores
dc.subjectInoculation
dc.subjectPathogen
dc.titleFirst report of rust caused by Phakopsora pachyrhizi on soybean in Democratic Republic of Congo
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationAvenue Isiro, Commune de la Gombe
cg.contributor.affiliationForeign Disease-Weed Science Research Unit
cg.contributor.affiliationUniversity of Illinois
cg.coverage.regionAfrica
cg.coverage.regionAcp
cg.coverage.regionWest Africa
cg.coverage.regionCentral Africa
cg.coverage.regionNorth America
cg.coverage.countryNigeria
cg.coverage.countryCongo, Dr
cg.coverage.countryUnited States
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Production
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectNutrition
cg.iitasubjectSoybean
cg.iitasubjectFood Security
cg.iitasubjectDiseases Control
cg.iitasubjectGenetic Improvement
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Diseases
cg.iitasubjectLivelihoods
cg.accessibilitystatusLimited Access
local.dspaceid95445
cg.identifier.doihttps://doi.org/10.1094/PDIS-91-9-1204C


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