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Development of a semi selective medium for isolation of Xanthomonas campestris pv. musacearum from banana plants

dc.contributor.authorTripathi, L.
dc.contributor.authorTripathi, J.N.
dc.contributor.authorTushemereirwe, W.K.
dc.contributor.authorBandyopadhyay, Ranajit
dc.date.accessioned2019-12-04T11:19:32Z
dc.date.available2019-12-04T11:19:32Z
dc.date.issued2007
dc.identifier.citationTripathi, L., Tripathi, J.N., Tushemereirwe, W.K. & Bandyopadhyay, R. (2007). Development of a semi-selective medium for isolation of Xanthomonas campestris pv. musacearum from banana plants. European Journal of Plant Pathology, 117(2), 177-186.
dc.identifier.issn0929-1873
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3483
dc.description.abstractBanana Xanthomonas wilt, caused by Xanthomonas campestris pv. musacearum, is a new threat to banana cultivation in eastern Africa. The causal bacterium grows slowly in culture and is easily overgrown by contaminants. A selective culture medium for isolation of X. c. pv. musacearum will facilitate disease study. A medium that suppressed saprophytic growth and possessed diagnostic characters for the pathogen was developed. Various carbon sources were tested with two isolates of X. c. pv. musacearum, and sucrose was selected as main carbon source. The susceptibility of X. c. pv. musacearum and other bacterial strains was tested with 29 different antibiotics. Cephalexin and cycloheximide had no effect on X. c. pv. musacearum but cephalexin inhibited most of the saprophytes and cycloheximide inhibited the fungal contaminants. Based on these studies, we have developed a semi-selective medium YTSA-CC containing yeast extract (1%), tryptone (1%), sucrose (1%), agar (1.5%), cephalexin (50 mg l−1) and cycloheximide (150 mg l−1), pH 7.0. The pathogen X. c. pv. musacearum was easily identified as yellowish, mucoid and circular colonies on YTSA-CC medium. This simple semi-selective medium was effective for isolation of X. c. pv. musacearum from infected banana tissues and soil, and it should be a valuable tool in ecological and epidemiological studies.
dc.description.sponsorshipGatsby Charitable Foundation
dc.language.isoen
dc.subjectAntibiotic Susceptibility
dc.subjectBanana Xanthomonas
dc.subjectWilt Carbon Sources
dc.subjectSemi-Selective Medium
dc.subjectXanthomonas Campestris Pv. Musacearum
dc.subjectPathogen
dc.subjectSaprophytic
dc.titleDevelopment of a semi selective medium for isolation of Xanthomonas campestris pv. musacearum from banana plants
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationKawanda Agricultural Research Institute, Uganda
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.regionWest Africa
cg.coverage.countryUganda
cg.coverage.countryNigeria
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectBanana
cg.iitasubjectFarm Management
cg.iitasubjectClimate Change
cg.iitasubjectDisease Control
cg.iitasubjectPlant Production
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.accessibilitystatusLimited Access
local.dspaceid95454
cg.identifier.doihttps://doi.org/10.1007/s10658-006-9083-7


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