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VNTRbased diversity analysis of 2x and 4x fullsib Musa hybrids

dc.contributor.authorCrouch, J.
dc.contributor.authorCrouch, H.
dc.contributor.authorTenkouano, A.
dc.contributor.authorOrtiz, R.
dc.date.accessioned2019-12-04T11:21:03Z
dc.date.available2019-12-04T11:21:03Z
dc.date.issued1999
dc.identifier.citationCrouch, J., Crouch, H., Tenkouano, A. & Ortiz, R. (1999). VNTR-based diversity analysis of 2x and 4x full-sib Musa hybrids. Electronic Journal of Biotechnology, 2(3), 1-11.
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3646
dc.description.abstractThe triploid plantain landrace Obino l Ewai (Musa spp., AAB genome) has been crossed with a wild diploid banana (M. acuminata subsp. burmannica var. Calcutta 4 , AA genome) to generate full-sib diploid and tetraploid hybrids combining good agronomic performance and disease resistance. Microsatellite marker analysis of the parental genotypes confirmed the highly heterozygous nature of both parental genotypes. Comparative analysis of 2x and 4x full-sib hybrids with their parental genotypes indicated that tetraploid hybrids are generally more closely related to Obino l Ewai than their diploid full-sibs. Based on VNTR analysis it is possible to identify those hybrids, which may be most useful in subsequent breeding of secondary triploid hybrids. There was a significant (P<0.05) negative association between the VNTR-based genetic similarity of hybrids to Obino l Ewai and a phenotypic distance index based on eight agronomic descriptors. However, there was no association between the molecular genetic similarity of hybrids to Calcutta 4 and the respective phenotypic distance index. Many microsatellite markers generated an unexpectedly high number of amplification products from AA and AAB genotypes plus their progeny which may suggest the presence of a high frequency of loci duplication in both A and B genomes, in addition to the detection of heterozygous and/or homoeologous loci.
dc.language.isoen
dc.subjectVntr
dc.subjectMusa
dc.subjectHybrids
dc.subjectDiversity
dc.subjectBreeding
dc.subjectParental Genotypes
dc.subjectTetraploid Hybrids
dc.subjectPhenotypic
dc.titleVNTRbased diversity analysis of 2x and 4x fullsib Musa hybrids
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR single centre
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectSoil Fertility
cg.iitasubjectSoil Information
cg.iitasubjectSoil Health
cg.iitasubjectPlant Diseases
cg.iitasubjectPests Of Plant
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Production
cg.iitasubjectFarm Management
cg.iitasubjectFood Security
cg.iitasubjectMarkets
cg.iitasubjectGenetic Improvement
cg.accessibilitystatusOpen Access
local.dspaceid95801


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