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    Using isozyme polymorphism to assess genetic variation within cultivated yams (Dioscorea cayenensis/Dioscorea rotundata complex) of the Republic of Benin

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    Date
    2000
    Author
    Dansi, A.
    Mignouna, H.
    Zoundjihékpon, J.
    Sangare, A.
    Asiedu, Robert
    Ahoussou, N.
    Type
    Journal Article
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    Abstract/Description
    Four hundred and sixty-seven accessions of cultivated Guinea yam (Dioscorea cayenensis/Dioscorea rotundatacomplex) collected from different localities of Benin Republic were analysed to study isoenzymatic variability in seven enzyme systems: aspartate aminotransferase (AAT), esterase (EST), glucose-6-phosphate dehydrogenase (G6PDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), phosphoglucoisomerase (PGI), and shikimate dehydrogenase (SKDH) using starch gel electrophoresis. Polymorphism was observed in all of the enzyme systems and a total of 62 electromorphs of different frequency and variability patterns were recorded. Different combinations of banding patterns of these systems led to identification of 227 different cultivars within the 467 accessions analysed. For an old and vegetatively propagated crop (with a considerable number of vernacular names) such as yam, and for which a high rate of duplication is expected, the 227 cultivars were found to be good enough to be considered as the adequate number of accessions representing the diversity in the germplasm analysed. Cluster analysis (UPGMA) produced a most likely division of the 467 accessions into two groups corresponding to D. rotundata Poir. and D. cayenensis Lam., supporting the concept that the two forms of guinea yam represent different genetic entities. The different clusters formed within the white yams (D. rotundata) did not exactly conform to the known cultivar groups. Additional polymorphic enzymes are needed for an accurate isozyme-based genetic discrimination of most of the cultivar groups.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3671
    IITA Subjects
    Yam; Farm Management; Diseases Control; Pests Of Plant; Capacity Development; Livelihoods; Plant Breeding; Handling, Transport, Storage And Protection Of Agricultural Products; Plant Production; Plant Diseases
    Agrovoc Terms
    Core Collection; Cultivar Groups; Dioscorea Cayenensis; Dioscorea Rotundata; Genetic Relatedness; Guinea Yam; Isozyme Markers; Yam Identification; Morphotype
    Regions
    Africa; West Africa
    Countries
    Benin; Nigeria; Cote D'Ivoire
    Collections
    • Journal and Journal Articles4835
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