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    Efficacy of chemical and flourescent protein markers in studying plant colonization by endophytic nonpathogenic Fusarium oxysporum isolates

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    Date
    2009
    Author
    Paparu, P.
    Macleod, A.
    Dubois, T.
    Coyne, D.L.
    Viljoen, A.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    In studying plant colonization by inoculated Fusarium oxysporum endophytes, it is important to be able to distinguish inoculated isolates from saprophytic strains. In the current study, F. oxysporum isolates were transformed with the green (GFP) and red fluorescent protein (DsRed) genes, and benomyl- and chlorate-resistant mutant isolates were also developed. The benomyl- and chlorate-resistant mutants, and the fluorescently labelled transformants, were able to grow on potato dextrose agar amended with 20 mg Benlate® l−1, 30 g chlorate l−1 and 150 μg hygromycin ml−1, respectively. Single spores of all mutants remained stable after several transfers on non-selective media. Most mutants and transformants produced colony diameters that did not differ significantly from that of their wild-type progenitors after 7 days of growth on non-selective media. Few mutants, however, had growth rates that were either slower or faster than for their wild-types. Plant colonization studies showed that root and rhizome tissue colonization by most benomyl- and chlorate-resistant mutants was similar to that of their wild-type isolates. Unlike GFP transformants, DsRed transformants were difficult to visualize in planta. Both the mutants and transformants can be used for future studies to investigate colonization, distribution and survival of biocontrol F. oxysporum endophytes in banana plants.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3824
    IITA Subjects
    Plant Diseases; Plant Health; Plantain; Banana
    Agrovoc Terms
    Bananas; Fusarium Oxysporum; Endophytes; Radopholus Similis; Chlorates; Proteins
    Regions
    Africa; East Africa
    Countries
    Uganda
    Collections
    • Journal and Journal Articles4835
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