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Genetic loci controlling carotenoid biosynthesis in diverse tropical maize lines

dc.contributor.authorAzmach, G.
dc.contributor.authorMenkir, A.
dc.contributor.authorSpilane, C.
dc.contributor.authorGedil, M.
dc.date.accessioned2019-12-04T11:21:38Z
dc.date.available2019-12-04T11:21:38Z
dc.date.issued2018-01-29
dc.identifier.citationAzmach, G., Menkir, A., Spillane, C. & Gedil, M. (2018). Genetic loci controlling carotenoid biosynthesis in diverse tropical maize lines. G3: Genes, Genomes, Genetics, 8(3), 1049-1065.
dc.identifier.issn2160-1836
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3838
dc.descriptionOpen Access Journal
dc.description.abstractThe discovery and use of genetic markers associated with carotenoid levels can help to exploit the genetic potential of maize for provitamin A accumulation more effectively. Provitamin A carotenoids are classes of carotenoids that are precursors of vitamin A, an essential micronutrient in humans. Vitamin A deficiency is a global public health problem affecting millions of people, especially in developing countries. Maize is one of the most important staple crops targeted for provitamin A biofortification to help alleviate vitamin A efficiency in developing countries. A genome-wide association study (GWAS) of maize endosperm carotenoids was conducted using a panel of 130 diverse yellow maize tropical inbred lines genotyped with Genotyping by Sequencing (GBS) SNP markers. Numerous significant association signals co-localizing with the known carotenoid biosynthesis genes crtRB1, lcyE and ZEP1 were identified. The GWAS confirmed previously reported large effects of the two major carotenoid biosynthesis genes lcyE and crtRB1. In addition, significant novel associations were detected for several transcription factors (e.g., RING zinc finger domain and HLH DNA-binding domain super family proteins) that may be involved in regulation of carotenoid biosynthesis in maize. When the GWAS was re-conducted by including the major effects of lcyE and crtRB1 genes as covariates, a SNP in a gene coding for an auxin response factor 20 transcription factor was identified which displayed an association with b-carotene and provitamin A levels. Our study provides a foundation for design and implementation of genomics-assisted selection strategies for provitamin A maize breeding in tropical regions, and advances efforts toward identification of additional genes (and allelic variants) involved in the regulation of carotenoid biosynthesis in plants.
dc.description.sponsorshipIrish Aid
dc.format.extent1049-1065
dc.language.isoen
dc.subjectProvitamins
dc.subjectMaize
dc.subjectVitamin A Deficiency
dc.subjectLinkage Disequilibriumline
dc.subjectMixed Linear Model
dc.subjectGenotyping By Sequencing
dc.subjectBiofortification Maize
dc.titleGenetic loci controlling carotenoid biosynthesis in diverse tropical maize lines
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNational University of Ireland
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.creator.identifierAbebe Menkir: 0000-0002-5907-9177
cg.creator.identifierMelaku Gedil: 0000-0002-6258-6014
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectMaize
cg.journalG3: Genes Genomes Genetics
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid96078
cg.targetaudienceScientists
cg.identifier.doihttps://doi.org/10.1534/g3.117.300511


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