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dc.contributor.authorNdungu, S.M.
dc.contributor.authorMessmer, M.M.
dc.contributor.authorZiegle, D.
dc.contributor.authorThuita, M.
dc.contributor.authorVanlauwe, Bernard
dc.contributor.authorFrossard, E.
dc.contributor.authorThonar, C.
dc.date.accessioned2019-12-04T11:21:38Z
dc.date.available2019-12-04T11:21:38Z
dc.date.issued2018-06
dc.identifier.citationNdungu, S.M., Messmer, M.M., Ziegler, D., Thuita, M., Vanlauwe, B., Frossard, E. & Thonar, C. (2018). Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya. Applied Microbiology and Biotechnology, 1-14.
dc.identifier.issn0175-7598
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3839
dc.descriptionPublished online: 25 April 2018
dc.description.abstractCowpea N2 fixation and yield can be enhanced by selecting competitive and efficient indigenous rhizobia. Strains from contrasting agro-ecologies of Kilifi and Mbeere (Kenya) were screened. Two pot experiments were established consisting of 13 Bradyrhizobium strains; experiment 1 (11 Mbeere + CBA + BK1 from Burkina Faso), experiment 2 (12 Kilifi + CBA). Symbiotic effectiveness was assessed (shoot biomass, SPAD index and N uptake). Nodule occupancy of 13 simultaneously co-inoculated strains in each experiment was analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to assess competitiveness. Strains varied in effectiveness and competitiveness. The four most efficient strains were further evaluated in a field trial in Mbeere during the 2014 short rains. Strains from bacteroids of cowpea nodules from pot and field experiments were accurately identified as Bradyrhizobium by MALDI-TOF based on the SARAMIS™ database. In the field, abundant indigenous populations 7.10 × 103 rhizobia g−1 soil, outcompeted introduced strains. As revealed by MALDI-TOF, indigenous strains clustered into six distinct groups (I, II, III, IV, V and VI), group III were most abundant occupying 80% of nodules analyzed. MALDI-TOF was rapid, affordable and reliable to identify Bradyrhizobium strains directly from nodule suspensions in competition pot assays and in the field with abundant indigenous strains thus, its suitability for future competition assays. Evaluating strain competitiveness and then symbiotic efficacy is proposed in bioprospecting for potential cowpea inoculant strains.
dc.format.extent5265-5278
dc.language.isoen
dc.subjectBradyrhizobium
dc.subjectCowpeas
dc.subjectNodulation
dc.subjectProteins
dc.subjectBacteroid
dc.titleEvaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.crpGrain Legumes
cg.contributor.affiliationInstitute of Agricultural Sciences, Switzerland
cg.contributor.affiliationResearch Institute of Organic Agriculture, Switzerland
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMabritec AG, Switzerland
cg.contributor.affiliationUniversité de Liège
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryKenya
cg.creator.identifierMoses Thuita: 0000-0002-6731-9492
cg.creator.identifierbernard vanlauwe: 0000-0001-6016-6027
cg.researchthemeNATURAL RESOURCE MANAGEMENT
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectCowpea
cg.iitasubjectGrain Legumes
cg.iitasubjectNatural Resource Management
cg.journalApplied Microbiology and Biotechnology
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid96092
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1007/s00253-018-9005-6


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