• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Identification and distribution of microsymbionts associated with soybean nodulation in Mozambican soils

    Thumbnail
    View/Open
    U18ArtGyogluuIdentificationInthomDev.pdf (1.307Mb)
    Date
    2018-05-20
    Author
    Gyogluu, C.
    Jaiswal, S.K.
    Boahen, S.
    Dakora, F.D.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Indigenous soybean rhizobial strains were isolated from root nodules sampled from farmers’ fields in Mozambique to determine their identity, distribution and symbiotic relationships. Plant infection assays revealed variable nodulation and symbiotic effectiveness among the 43 bacterial isolates tested. Strains from Ruace generally promoted greater whole-plant growth than the others. 16S rRNA-RFLP analysis of genomic DNA extracted from the rhizobial isolates produced different banding patterns, a clear indication of high bacterial diversity. However, the multilocus sequence analysis (MLSA) data showed alignment of the isolates with B. elkanii species. The 16S rRNA sequences of representative soybean isolates selected from each 16S rRNA-RFLP cluster showed their relatedness to B. elkanii, as well as to other Bradyrhizobium species. But a concatenated phylogeny of two housekeeping genes (glnII and gyrB) identified the soybean nodulating isolates as Bradyrhizobium, with very close relatedness to B. elkanii. The nifH and nodC sequences also showed that the majority of the test soybean isolates were closely related to B. elkanii, albeit the inconsistency with some isolates. Taken together, these findings suggest that the B. elkanii group are the preferred dominant microsymbiont of soybean grown in Mozambican soils. Furthermore, the distribution of soybean rhizobia in the agricultural soils of Mozambique was found to be markedly influenced by soil pH, followed by the concentrations of plant-available P and Mn. This study suggested that the identified isolates TUTMJM5, TUTMIITA5A and TUTLBC2B can be used as inoculants for increased soybean production in Mozambique.
    https://dx.doi.org/10.1016/j.syapm.2018.05.003
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3864
    Non-IITA Authors ORCID
    Stephen Boahen Asaberehttps://orcid.org/0000-0001-8946-401X
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1016/j.syapm.2018.05.003
    IITA Subjects
    Grain Legumes; Plant Diseases; Plant Genetic Resources; Soybean
    Agrovoc Terms
    Phylogeny; Biogeography; Mozambique; Root Nodulation; Soybeans; Dna
    Regions
    Africa; Southern Africa
    Countries
    Mozambique
    Journals
    Systematic and Applied Microbiology
    Collections
    • Journal and Journal Articles4836
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository