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dc.contributor.authorHughes, J.
dc.contributor.authorDongo, L.
dc.contributor.authorNg, S.Y.C.
dc.date.accessioned2019-12-04T11:22:06Z
dc.date.available2019-12-04T11:22:06Z
dc.date.issued1998
dc.identifier.citationHughes, J., Dongo, L. & Ng, S.Y.C. (1998). Diagnosis of yam viruses. Tropical Agriculture, 75(1), 45-48.
dc.identifier.issn0041-3216
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3943
dc.description.abstractDiagnosis of virus diseases of yams at the International Institute of Tropical Agriculture (IITA) is based on the use of indicator plants, insect vectors, serological diagnostics, and electron microscopy (EM). Yam I (YVI), yam mosaic (YMV), and Dioscorea dumetorum (DdV) potyviruses even in mixed virus infection. Yam mosaic virus is mechanically transmissible to Nicotiana benthamiana and YVI is vector-transmissible to Vigna unguiculata. The three viruses can be visualised by EM and their identities confirmed serologically. Dioscorea alata badnavirus (DaV) can be graft-transmitted to V, unguiculata and its presence confirmed by EM and serology, Cucumber mosaic cucumovirus (CMV), reported to occur in yams in West is readily mechanically transmissible to a range of indicator plants and can be detected serologically. Other isometric viruses have recently been found in Nigeria, and these can be isolated and differentiated by mechanical and vector transmission to indicator plants. These diagnostic techniques have enabled initiation of surveys of viruses of Dioscorea spp. in West Africa and substantial progress has been made on the production of virus-tested planting material for international distribution.
dc.language.isoen
dc.subjectDioscorea
dc.subjectPlant Viruses
dc.subjectDiagnosis
dc.subjectElisa
dc.subjectMicroscopy
dc.titleDiagnosis of yam viruses
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR single centre
cg.iitasubjectYam
cg.iitasubjectPlant Diseases
cg.iitasubjectCowpea
cg.accessibilitystatusLimited Access
local.dspaceid99229


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