• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Studies on a Nigerian isolate of banana streak badnavirus: II. Effect of intraplant variation on virus accumulation and reliability of diagnosis by ELISA

    Thumbnail
    View/Open
    S98ArtDahalStudiesInthomNodev.PDF (985.9Kb)
    Date
    1998
    Author
    Dahal, G.
    Pasberg-Gauhl, C.
    Gauhl, F.
    Thottappilly, G.
    Hughes, J.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    Monitoring of banana streak badnavirus (BSV) antigens and symptoms in naturally BSV‐infected plantain and banana (Musa spp.) plants showed a great variation in symptom expression, distribution and relative concentration of BSV between and within plants. Expression and distribution of symptoms was erratic within individual leaves as well as between different leaves of the same plant. The concentration of BSV antigens detected by triple antibody sandwich enzyme linked immunosorbent assay (TAS‐ELISA) varied in different plant parts including leaf lamina, midrib and pseudostem, roots and young ‘cigar' leaf. The concentration of BSV antigens was high in symptomatic tissues but was low or below the limits of detection in most asymptomatic tissues. During ‘hot dry' seasons when symptoms were not fully expressed, the concentration of BSV antigens in leaf tissues declined drastically, often below the detection limit of TAS‐ELISA. These results suggested that for more reliable detection of BSV antigens by TAS‐ELISA, it is advisable to index plants using composite tissue samples comprising as many leaves as possible for each plant and collected during cool and/or rainy seasons when symptom expression is generally severe.
    https://doi.org/10.1111/j.1744-7348.1998.tb05202.x
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3952
    Digital Object Identifier (DOI)
    https://doi.org/10.1111/j.1744-7348.1998.tb05202.x
    IITA Subjects
    Banana; Plant Diseases; Plantain
    Agrovoc Terms
    Banana Streak Virus; Musabadnavirus; Badnavirus; Virus Distribution
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository