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    Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population

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    akano-application-1998.pdf (549.2Kb)
    Date
    1998
    Author
    Akano, A.O.
    Ng, S.Y.C.
    Dixon, A.
    Thottappilly, G.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    In a study to map out the genes confering resistanceto African cassava msaic disease (ACMD), a cost-effective protocol for zygotic embryo culture developed at the International Institute of Tropical Agriculture(IITA) using 1/2 strenght Murashige and Skoog's basal media, was employed to germinatethe F1 populations. The F1 crosses wee generated from cross combinations between ACMD resistant and susceptible and local cassava germplasm. These crosses were TMS 30572 x TME 117, TMS 30572 x TME 4, and TMS 30555 x TME 3, giving three mapping populations. Four weeks after explanting embryo axes, progenies of the F1 cross of TMS 30555 x TME 3 perfomed best with 88% of the explanted embryos poducing two or more nodes, followed by those of the F1 cross TMS 30572 x TME 4, with 79%, and TMS 30572 x TME 117, germilings had 4 to 5 nodes, and 3, 5, and 9% in F1 crosses of TMS 30572 x TME 117, TMS 30572 x TME 4, and TMS 30555 X tme 3, respectively.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3961
    IITA Subjects
    Cassava; Plant Diseases; Genetic Improvement; Soil Surveys And Mapping
    Agrovoc Terms
    African Cassava Mosaic Virus; Zygotic Embryo; Embryo Axes; Mapping Population
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles4835
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