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Comparison of PCRbased molecular marker analyses of Musa breeding populations

dc.contributor.authorCrouch, J.H.
dc.contributor.authorCrouch, H.K.
dc.contributor.authorConstandt, H.
dc.contributor.authorGysel, A. van
dc.contributor.authorBreyne, P.
dc.contributor.authorMontagu, M. van
dc.contributor.authorJarret, R.L.
dc.contributor.authorOrtiz, R.
dc.date.accessioned2019-12-04T11:22:26Z
dc.date.available2019-12-04T11:22:26Z
dc.date.issued1999
dc.identifier.citationCrouch, J.H., Crouch, H.K., Constandt, H., Van Gysel, A., Breyne, P., Van Montagu, M., ... & Ortiz, R. (1999). Comparison of PCR-based molecular marker analyses of Musa breeding populations. Molecular Breeding, 5(3), 233-244.
dc.identifier.issn1380-3743
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4006
dc.description.abstractProgress in the breeding of plantain and banana has been restricted by the complex genetic structure and behaviour of cultivated polyploid Musa. Genetic improvement has been hindered due to the large amount of space required for growth and maintenance of plant populations, in addition to the long growth cycle and the low levels of fertility and seed viability characteristic of cultivated genotypes. Molecular marker assisted breeding has the potential to dramatically enhance the pace and efficiency of genetic improvement in Musa. This study was conducted to compare different PCR-based marker systems (RAPD, VNTR and AFLP) for the analysis of breeding populations generated from two diverse Musa breeding schemes. All three assays detected a high level of polymorphism between parental genotypes and within progeny populations. As expected, AFLP assays had by far the highest multiplex ratio while VNTR analysis detected the highest levels of polymorphism. AFLP analysis of a full-sib tetraploid hybrid population confirmed previous reports based on VNTR analysis, of a high frequency of recombination during 2n (3x) gamete formation by a triploid plantain landrace. In addition, both VNTR and RAPD analyses of a full-sib triploid hybrid population suggested a high frequency of homoeologous recombination during n (2x) gamete formation by tetraploid hybrids. In general, there was a poor correlation between estimates of genetic similarity based on different types of marker. The implications of these findings for the molecular breeding of Musa crops are discussed.
dc.description.sponsorshipOverseas Development Administration, United Kingdom
dc.language.isoen
dc.subjectPolymerase Chain Reaction
dc.subjectRandom Amplified Polymorphic
dc.subjectMusa
dc.subjectPlantains
dc.subjectRapd
dc.subjectAflp
dc.subjectBreeding
dc.subjectGenetic Improvement
dc.titleComparison of PCRbased molecular marker analyses of Musa breeding populations
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Gent
cg.contributor.affiliationUnited States Department of Agriculture
cg.contributor.affiliationRoyal Veterinary and Agricultural University, Denmark
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlantain
cg.iitasubjectBanana
cg.iitasubjectPlant Breeding
cg.accessibilitystatusLimited Access
local.dspaceid99292


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