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    Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa

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    S98ArtOsujiPloidyInthomNodev.PDF (437.0Kb)
    Date
    1998
    Author
    Osuji, J.O.
    Vuylsteke, D.
    Ortiz, R.
    Type
    Journal Article
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    Abstract/Description
    Hybrids were obtained after in vitro germination of embryos from interploid crosses between triploid ‘French' plantain cultivars (Musa spp. AAB group). ‘Ntanga 2' and ‘Bobby Tannap' with diploid banana (Musa acuminata subsp. burmannicoides) ‘Calcutta 4'. Cross-pollinated bunches were harvested at full maturity and ripened with acetylene in a room for 4 days. Seeds were extracted from peeled ripe fruits by squashing. Embryos from the seeds were excised aseptically after 2 days and germinated in vitro. Seedlings were subsequently planted in early evaluation trials after acclimatizing in the greenhouse. Chromosome counts were carried out on root tips of mature and maiden suckers to determine ploidy levels using a modified squashing technique. Counts showed that two of the hybrids were aneuploids (trisomics) with somatic chromosome number of 2n = 2x + 1 = 23. One hybrid was diploid while the other two were tetraploids. Tetraploids are the most promising hybrids for the genetic improvement of plantains. Diploids are valuable material for further improvement of the plantain genome at this ploidy level. Trisomics provide means for further characterization of the Musa genome and physical gene mapping in plantain and banana.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/4008
    IITA Subjects
    Banana; Plant Breeding; Plantain; Genetic Improvement
    Agrovoc Terms
    Aneuploids; Bananas; Breeding; Cytogenetics; Plantains; Ploidy Manipulation
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles4835
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