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Identification of the genomic constitution of Musa L. lines (bananas, plantains and hybrids) using molecular cytogenetics

dc.contributor.authorOsuji, J.
dc.contributor.authorHarrison, G.
dc.contributor.authorCrouch, J.
dc.contributor.authorHeslop-Harrison, J.S.
dc.date.accessioned2019-12-04T11:23:37Z
dc.date.available2019-12-04T11:23:37Z
dc.date.issued1997
dc.identifier.citationOsuji, J., Harrison, G., Crouch, J. & Heslop-Harrison, J. (1997). Identification of the genomic constitution of Musa L. lines (bananas, plantains and hybrids) using molecular cytogenetics. Annals of Botany, 80, 787-793.
dc.identifier.issn0305-7364
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4066
dc.description.abstractThe genomic constitutions of some Musa L. lines (bananas, plantains and artificial hybrids) were identified using molecular cytogenetic techniques. Double target in situ DNA:DNA hybridization to chromosome spreads using as probes, total genomic DNA isolated from diploid Musa lines of known AA (labelled with biotin-11-dUTP) and BB (labelled with digoxigenin-11-dUTP) genome constitution was carried out. The use of 60% acetic acid combined with heating over a flame gave high quality chromosome spreads free of cytoplasm for in situ hybridization. Total genomic A DNA labelled broad centromeric regions of all 22 chromosomes of the diploid line, Calcutta 4 ( M. acuminata Colla. ssp. burmanniccoides ; A genome) with some chromosomes showing stronger hybridization. Labelled DNA from the B genome hybridized strongly to the centromeric regions of all 22 chromosomes of Butohan 2 ( M. balbisiana Colla; B genome). The two satellited chromosomes of genome B labelled strongly with genomic A DNA. In situ hybridization of labelled A and B genomic DNA to metaphase chromosomes of triploid AAB and ABB cultivars discriminated between A and B genome chromosomes. The plantains Agbagba, Obino l'Ewai and Mbi Egome showed 22 genome A and 11 genome B chromosomes while the cooking bananas Bluggoe and Fougamou showed 11 genome A and 22 genome B chromosomes. Hybridization of labelled A and B genomic DNA to chromosomes of the hybrids showed that TMP2x 2829-62 has all 22 genome A chromosomes while TMPx 4698-1 has 33 genome A and 11 genome B chromosomes. In situ hybridization of labelled total genomic DNA to chromosomes has immense potential for identification of chromosome origin and can be used to characterize cultivars and hybrids produced in Musa breeding.
dc.language.isoen
dc.subjectGenomic In Situ Hybridization
dc.subjectBananas
dc.subjectPlantains
dc.subjectHybrids
dc.subjectPlant Breeding
dc.subjectGenome Organization
dc.subjectBiodiversity
dc.titleIdentification of the genomic constitution of Musa L. lines (bananas, plantains and hybrids) using molecular cytogenetics
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversity of Leicester
cg.contributor.affiliationUniversity of Port Harcourt
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionEurope
cg.coverage.regionWest Africa
cg.coverage.countryUnited Kingdom
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectBanana
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPlant Production
cg.iitasubjectPlant Diseases
cg.iitasubjectPests Of Plants
cg.iitasubjectFarm Management
cg.iitasubjectDisease Control
cg.iitasubjectLivelihoods
cg.iitasubjectGenetic Improvement
cg.iitasubjectBiodiversity
cg.iitasubjectPlantain
cg.accessibilitystatusLimited Access
local.dspaceid99352
cg.identifier.doihttps://doi.org/10.1006/anbo.1997.0516


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