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dc.contributor.authorBoukar, O.
dc.contributor.authorKong, L.
dc.contributor.authorSingh, B.B.
dc.contributor.authorMurdock, L.
dc.contributor.authorOhm, H.W.
dc.date.accessioned2019-12-04T11:23:46Z
dc.date.available2019-12-04T11:23:46Z
dc.date.issued2004
dc.identifier.citationBoukar, O., Kong, L., Singh, B.B., Murdock, L. & Ohm, H.W. (2004). AFLP and AFLP-derived SCAR markers associated with Striga gesnerioides resistance in cowpea. Crop Science, 44(4), 1259-1264.
dc.identifier.issn0011-183X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4146
dc.description.abstractCowpea, Vigna unguiculata (L.) Walp., is an important grain legume grown in tropical and subtropical regions, primarily Africa. The parasitic weed Striga gesnerioides (Willd.) Vatke is one of the most important constraints to cowpea production. Host plant resistance is the only practical control method. Five virulence genotypes (races) of S. gesnerioides have been identified in different regions of Africa. Several host resistance genes have also been identified that are effective against specific races of S. gesnerioides The rapid spread of this parasitic weed creates an urgent need for cowpea varieties with multiple resistance genes. A recently identified cowpea breeding line, IT93K-693-2, has resistance to all known races. The objective of this research was to develop DNA markers that are useful for marker-assisted selection (MAS) in breeding cowpea for resistance to S. gesnerioides An F2 population developed from the cross between IT93K-693-2 and the susceptible cultivar IAR1696 was characterized for resistance against race 3 of S. gesnerioides for genetic analysis and molecular mapping. IT93K-693-2 was found to have a single dominant gene for resistance. Four amplified fragment length polymorphism (AFLP) markers, designated E-ACT/M-CTC115, E-ACT/M-CAC115, E-ACA/M-CAG108 and E-AAG/E-CTA190, were identified and mapped 3.2, 4.8, 13.5 and 23.0 cM, respectively, from Rsg1, a gene in IT93K-693-2 that gives resistance to race 3 (or Nigerian strain) of S. gesnerioides The first two markers were validated in a second F2 population developed from crossing the same resistant parent with ‘Kamboinse local’, a different susceptible cultivar. The AFLP fragment from marker combination E-ACT/M-CAC, which is linked in coupling with Rsg1 was cloned, sequenced, and converted into a sequence characterized amplified region (SCAR) marker named SEACTMCAC83/85, which is codominant and useful in breeding programs.
dc.language.isoen
dc.subjectCowpeas
dc.subjectStriga Gesnerioide
dc.subjectAflp
dc.titleAFLP and AFLPderived SCAR markers associated with Striga gesnerioides resistance in cowpea
dc.typeJournal Article
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationPurdue University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectCowpea
cg.accessibilitystatusLimited Access
local.dspaceid99712
cg.identifier.doihttps://doi.org/10.2135/cropsci2004.1259


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