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    Establishing the genome of Sukali ndizi

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    Date
    2003
    Author
    Pillay, M.
    Hartman, J.
    Dimkpa, C.O.
    Makumbi, Dan
    Type
    Journal Article
    Journal Article
    Metadata
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    Abstract/Description
    Cultivated bananas originated from interspecific hybridisation of two wild diploid (2n = 2x = 22) species, Musa acuminata and M. balbisiana that were the donors of the A and B genomes, respectively. Most cultivated bananas are triploids with 2n = 3x = 33 chromosomes. They vary in genome composition with the most common types being AAA (dessert), AAB (plantain) and ABB (cooking banana). Banana improvement programs make use of interspecific hybridisation for gene introgression. Consequently knowledge of the exact ploidy level and genome composition of a plant is important for breeding purposes. The ploidy level of a plant is generally determined in two ways: (1) by a physical count of its chromosomes, and (2) by flow cytometry. In this study, the ploidy level of the dessert banana cultivar, 'Sukali Ndizi', was determined by conventional chromosome analysis and flow cytometry. Our results showed that 'Sukali Ndizi' is a triploid. In the past, 'Sukali Ndizi' was considered to be a diploid with an AB genome composition. We also determined the genome composition of the cultivar using a set of RAPD (random amplified polymorphic DNA) markers and found that it has an AAB genome composition.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/4182
    IITA Subjects
    Banana; Plant Genetic Resources; Genetic Improvement
    Agrovoc Terms
    Bananas; Interspecific Hybridization; Rapd; Genomes
    Regions
    Africa; East Africa
    Countries
    Uganda
    Collections
    • Journal and Journal Articles4835
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