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Resistance to aflatoxin accumulation in kernels of maize inbreds selected for ear rot resistance in West and Central Africa

dc.contributor.authorBrown, R.L.
dc.contributor.authorChen, Z.Y.
dc.contributor.authorMenkir, A.
dc.contributor.authorCleveland, T.E.
dc.contributor.authorCardwell, K.
dc.contributor.authorKling, J.
dc.contributor.authorWhite, D.G.
dc.date.accessioned2019-12-04T11:24:16Z
dc.date.available2019-12-04T11:24:16Z
dc.date.issued2001-03
dc.identifier.citationBrown, R.L., Chen, Z.Y., Menkir, A., Cleveland, T.E., Cardwell, K., Kling, J. & White, D.G. (2001). Resistance to aflatoxin accumulation in kernels of maize inbreds selected for ear rot resistance in West and Central Africa. Journal of Food Protection, 64(3), 396-400.
dc.identifier.issn0362-028X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4328
dc.description.abstractThirty-six inbred lines selected in West and Central Africa for moderate to high resistance to maize ear rot under conditions of severe natural infection were screened for resistance to aflatoxin contamination using the previously established kernel screening assay. Results showed that more than half the inbreds accumulated aflatoxins at levels as low as or lower than the resistant U.S. lines GT-MAS:gk or MI82. In 10 selected aflatoxin-resistant or aflatoxin-susceptible inbreds, Aspergillus flavus growth, which was quantified using an A. flavus transformant containing a GUS-β-tubulin reporter gene construct, was, in general, positively related to aflatoxin accumulation. However, one aflatoxin-resistant inbred supported a relatively high level of fungal infection, whereas two susceptibles supported relatively low fungal infection. When kernels of the 10 tested lines were profiled for proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, significant variations from protein profiles of U.S. lines were observed. Confirmation of resistance in promising African lines in field trials may significantly broaden the resistant germplasm base available for managing aflatoxin contamination through breeding approaches. Biochemical resistance markers different from those being identified and characterized in U.S. genotypes, such as ones inhibitory to aflatoxin biosynthesis rather than to fungal infection, may also be identified in African lines. These discoveries could significantly enhance the host resistance strategy of pyramiding different traits into agronomically useful maize germplasm to control aflatoxin contamination.
dc.format.extent396-400
dc.language.isoen
dc.subjectMaize
dc.subjectAflatoxins
dc.subjectGenotypes
dc.subjectResistance Varieties
dc.titleResistance to aflatoxin accumulation in kernels of maize inbreds selected for ear rot resistance in West and Central Africa
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.affiliationUnited States Department of Agriculture
cg.contributor.affiliationLouisiana State University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Illinois
cg.coverage.regionAfrica
cg.coverage.regionWest And Central Africa
cg.creator.identifierAbebe Menkir: 0000-0002-5907-9177
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAflatoxin
cg.iitasubjectMaize
cg.iitasubjectPlant Breeding
cg.journalJournal of Food Protection
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid99898
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.4315/0362-028X-64.3.396


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