• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Efficacy of chemotherapy and thermotherapy in elimination of east African cassava mosaic virus from Tanzanian cassava landrace

    Thumbnail
    View/Open
    U18ArtKidulileEfficacyInthomDev.pdf (714.3Kb)
    Date
    2018-08-12
    Author
    Kidulile, C.E.
    Ateka, E.M.
    Alakonya, A.
    Ndunguru, J.C.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Cassava mosaic disease is caused by cassava mosaic begomoviruses (CMBs) and can result in crop losses up to 100% in cassava (Manihot esculenta) in Tanzania. We investigated the efficacy of chemotherapy and thermotherapy for elimination of East African cassava mosaic virus (EACMV) of Tanzanian cassava. In vitro plantlets from EACMV‐infected plants obtained from coastal Tanzania were established in the greenhouse. Leaves were sampled from the plants and tested to confirm the presence of EACMV. Plantlets of plants positive for EACMV were initiated in Murashige and Skoog (MS) medium. On the second subculture, they were subjected into chemical treatment in the medium containing salicylic acid (0, 10, 20, 30 and 40 mg/L) and ribavirin (0, 5, 10, 15 and 20 mg/L). In the second experiment, EACMV‐infected plantlets were subjected to temperatures between 35 and 40°C with 28°C as the control. After 42 days of growth, DNA was extracted from plant leaves and PCR amplification was performed using EACMV specific primers. It was found that plant survival decreased with increasing levels of both salicylic acid and ribavirin concentrations. In general, plants treated with salicylic acid exhibited a lower plant survival % than those treated with ribavirin. However, the percentage of virus‐free plants increased with an increase in the concentration of both ribavirin and salicylic acid. The most effective concentrations were 20 mg/L of ribavirin and 30 mg/L of salicylic acid; these resulted in 85.0% and 88.9% virus‐free plantlets, respectively. With regard to thermotherapy, 35°C resulted in 79.5% virus‐free plantlets compared to 69.5% at 40°C. Based on virus elimination, ribavirin at 20 mg/L, salicylic acid 30 mg/L and thermotherapy at 35°C are recommended for production of EACMV free cassava plantlets from infected cassava landraces.
    https://dx.doi.org/10.1111/jph.12725
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/4377
    Non-IITA Authors ORCID
    amos Alakonyahttps://orcid.org/0000-0001-7710-499X
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1111/jph.12725
    Research Themes
    PLANT PRODUCTION & HEALTH
    IITA Subjects
    Plant Health; Plant Production
    Agrovoc Terms
    African Cassava Mosaic Virus; Begomovirus; Ribavirin; Salicylic Acids; Virus-Free Plants
    Regions
    Africa; East Africa
    Countries
    Tanzania
    Journals
    Journal of Plant Pathology
    Collections
    • Journal and Journal Articles4839
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository