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A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.

dc.contributor.authorNjukeng, A.P.
dc.contributor.authorAriri, G.I.
dc.contributor.authorHughes, Jacqueline d'Arros
dc.contributor.authorAgindoran, B.O.
dc.contributor.authorMignouna, H.D.
dc.contributor.authorThottappilly, G.
dc.date.accessioned2019-12-04T11:26:25Z
dc.date.available2019-12-04T11:26:25Z
dc.date.issued2002
dc.identifier.citationNjukeng, A.P., Atiri, G.I., D'A Hughes, J., Agindotan, B.O., Mignouna, H.D. & Thottappilly, G. (2002). A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. Tropical Science, 42(2), 65-74.
dc.identifier.issn0041-3291
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4596
dc.description.abstractA Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced by fusion of FOX-NY myeloma cells with immune splenocytes from in vivo immunizations of a Balb/C mouse. The polyclonal antiserum to YMV and the Mab (YMV-M24) were used in developing a triple antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) system for the detection of YMV from samples collected from fields. The test gave a positive reaction even at a sap dilution of 1:6250-1:31250 from leaf samples collected from different plants species, while antigen-coated plant (ACP) ELISA using the Mab could detect only to a dilution of 1:1250. TAS-ELISA was more sensitive and reliable when compared with ACP-ELISA using YMV-M24. YMV-M24 detected YMV isolates in some Dioscorea species from seven countries in the main yam growing area of West Africa.
dc.format.extent65-74
dc.language.isoen
dc.rightsCopyrighted; all rights reserved
dc.subjectYams
dc.subjectPlant Viruses
dc.subjectMonoclonal Antibodies
dc.subjectDioscorea
dc.titleA sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationUniversity of Dschang
cg.contributor.affiliationUniversity of Ibadan
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Diseases
cg.iitasubjectYam
cg.journalTropical Science
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid100878
cg.targetaudienceScientists


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