• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    First report of the presence of L1014S Knockdown-resistance mutation in Anopheles gambiae s.s and Anopheles coluzzii from Togo, West Africa

    Thumbnail
    View/Open
    U18ArtDjegbeFirstNothomDev.pdf (1.085Mb)
    Date
    2018
    Author
    Djegbe, I.
    Akoton, R.
    Tchigossou, G.
    Ahadji-Dabla, K.M.
    Atoyebi, S.M.
    Adeoti, R.
    Zeukeng, F.
    Ketoh, G.K.
    Djouaka, R.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Background: To optimize the success of insecticide-based malaria control intervention, knowledge of the distribution of Anopheles gambiae species and insecticide resistance mechanisms is necessary. This paper reported an updated data on pyrethroids/DDT resistance in the An. gambiae s.l population from Togo. Methods: From December 2013 to April 2015, females of indoor-resting An. gambiae s.l were captured in three locations belonging to three different ecological zones. Resistance to DDT, permethrin and deltamethrin was screened in F1 progeny of collected mosquitoes using WHO susceptibility tests. The identification of species of An. gambiae complex and the detection of kdr and ace.1 R allele were carried out using DNA-based molecular techniques. Results: An. gambiae from Kovié and Nangbéto were highly resistant to DDT and permethrin with mortalities rate ranging from 0.83% to 1.58% for DDT and zero to 8.54% for permethrin. Mosquitoes collected in Nangbéto displayed 81.53% mortality with deltamethrin. An. coluzzii and An. gambiae s.s were found in sympatry in Nangbéto and Mango . The allelic frequency of L1014F was high, ranging from 66 to 100% in both An. coluzzii and An. gambiae s.s. For the first time we detected the L1014S allele in both An. coluzzii and An. gambiae s.s. from Togo at the frequency ranging from 5% to 13% in all the sites. The kdr N1575Y was present at various frequencies in both species ranging from 10% to 45%. Both An. gambiae s.s. and An. coluzzii shared the ace1 R mutation in all investigated sites with allelic frequency ranging from 4% to 16%. Conclusion: These results showed that multiple mutations are involved in insecticides resistance in An. gambiae populations from Togo including the kdr L1014F, L1014S, and N1575Y and ace.1 R G119S mutations.
    https://dx.doi.org/10.12688/wellcomeopenres.13888.1
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/4613
    Non-IITA Authors ORCID
    Rousseau Djouakahttps://orcid.org/0000-0003-4772-0753
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.12688/wellcomeopenres.13888.1
    Research Themes
    NUTRITION & HUMAN HEALTH
    IITA Subjects
    Disease Control; Nutrition
    Agrovoc Terms
    Chemical Resistance; Anopheles Gambiae; Malaria; Disease Control; Togo; West Africa
    Regions
    Africa; West Africa
    Countries
    Togo
    Journals
    Wellcome Open Research
    Collections
    • Journal and Journal Articles4836
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository