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    Molecular and cytogenetic study of east African highland banana

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    U18ArtNemeckovaMolecularInthomNodev.pdf (4.232Mb)
    Date
    2018-10-04
    Author
    Nemecková, A.
    Christelová, P.
    Cížková, J.
    Nyine, M.
    Houwe, I. van den
    Svacina, R.
    Uwimana, B.
    Swennen, R.
    Dolezel, Jaroslav
    Hribova, E.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    East African highland bananas (EAHBs) are staple food crop in Uganda, Tanzania, Burundi, and other countries in the African Great Lakes region. Even though several morphologically different types exist, all EAHBs are triploid and display minimal genetic variation. To provide more insights into the genetic variation within EAHBs, genotyping using simple sequence repeat (SSR) markers, molecular analysis of ITS1-5.8S-ITS2 region of ribosomal DNA locus, and the analysis of chromosomal distribution of ribosomal DNA sequences were done. A total of 38 triploid EAHB accessions available in the Musa germplasm collection (International Transit Centre, Leuven, Belgium) were characterized. Six diploid accessions of Musa acuminata ssp. zebrina, ssp. banksii, and ssp. malaccensis representing putative parents of EAHBs were included in the study. Flow cytometric estimation of 2C nuclear DNA content revealed small differences (max ~6.5%) in genome size among the EAHB clones. While no differences in the number of 45S and 5S rDNA loci were found, genotyping using 19 SSR markers resulted in grouping the EAHB accessions into four clusters. The DNA sequence analysis of the internal transcribed spacer region indicated a relation of EAHB clones with M. acuminata and, surprisingly, also with M. schizocarpa. The results suggest that EAHB cultivars originated from a single hybrid clone with M. acuminata ssp. zebrina and ssp. banksii being its most probable parents. Howeve
    http://dx.doi.org/10.3389/fpls.2018.01371
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/4621
    Non-IITA Authors ORCID
    Rony Swennenhttps://orcid.org/0000-0002-5258-9043
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.3389/fpls.2018.01371
    Research Themes
    BIOTECH & PLANT BREEDING
    IITA Subjects
    Banana; Plant Breeding
    Agrovoc Terms
    Bananas; Musa; Dna; Fluorescence; Genotypes
    Regions
    Africa; East Africa
    Countries
    Burundi; Tanzania; Uganda
    Journals
    Frontiers in Plant Science
    Collections
    • Journal and Journal Articles4835
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