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An EST-SSR based genetic linkage map and identification of QTLs for anthracnose disease resistance in water yam (Dioscorea alata L.)

dc.contributor.authorBhattacharjee, R.
dc.contributor.authorNwadili, C.O.
dc.contributor.authorSaski, C.A.
dc.contributor.authorAgre, A.P.
dc.contributor.authorScheffle, B.E.
dc.contributor.authorAugusto, J.
dc.contributor.authorLópez Montes, A.
dc.contributor.authorOnyeka, J.T.
dc.contributor.authorKumar, P.L.
dc.contributor.authorBandyopadhyay, Ranajit
dc.date.accessioned2019-12-04T11:26:29Z
dc.date.available2019-12-04T11:26:29Z
dc.date.issued2018-10-10
dc.identifier.citationBhattacharjee, R., Nwadili, C.O., Saski, C.A., Agre, P., Scheffler, B.E., Augusto, J., ... & Bandyopadhyay, R. (2018). An EST-SSR based genetic linkage map and identification of QTLs for anthracnose disease resistance in water yam (Dioscorea alata L.). PLOS ONE, 13(10):318378, 1-16.
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4623
dc.descriptionOpen Access Journal
dc.description.abstractWater yam (Dioscorea alata L.) is one of the most important food yams with wide geographical distribution in tropics. One of the major constraints to water yam production is anthracnose disease caused by a fungus, Colletotrichum gloesporioides (Penz.). There are no economically feasible solutions as chemical sprays or cultural practices, such as crop rotation are seldom convenient for smallholder farmers for sustainable control of the disease. Breeding for development of durable genetic resistant varieties is known to offer lasting solution to control endemic disease threats to crop production. However, breeding for resistance to anthracnose has been slow considering the biological constraints related to the heterozygous and vegetative propagation of the crop. The development of saturated linkage maps with high marker density, such as SSRs, followed by identification of QTLs can accelerate the speed and precision of resistance breeding in water yam. A total of 380 EST-SSRs were used to generate a saturated linkage map. About 60.19% of SSRs showed Mendelian segregation pattern, however, it had no effect on the construction of linkage map. All 380 EST-SSRs were mapped into 20 linkage groups covering a total length of 2559.66 cM, which agrees with the diploid nature (2n = 2x = 20) of the parents used in the cross. Majority of the markers were mapped on linkage group 1 comprising of 97 EST-SSRs. This is the first genetic linkage map of water yam constructed using EST-SSRs. QTL localization was based on phenotypic data collected over a 3-year period of inoculating the mapping population with the most virulent strain of C. gloeosporoides from West Africa. Using the mean permutation value of LOD scores as threshold value for declaring a putative QTL on all linkage groups, one QTL was consistently observed on linkage group (LG) 14 in all the three years and average score data. This QTL was found at position interval of 71.12 - 84.76 cM explaining 68.94% of the total phenotypic variation in the average score data. The high marker density allowed identification of QTLs and association for anthracnose disease, which could be validated in other mapping populations and used in marker-assisted breeding in D. alata improvement programmes.
dc.description.sponsorshipUnited States Agency for International Development
dc.format.extent1-16
dc.language.isoen
dc.rightsCC0-1.0
dc.subjectColletotrichum Gloeosporioides
dc.subjectYams
dc.subjectQtl
dc.subjectWater Yam
dc.subjectDioscorea Alata
dc.titleAn EST-SSR based genetic linkage map and identification of QTLs for anthracnose disease resistance in water yam (Dioscorea alata L.)
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpMaize
cg.contributor.crpGrain Legumes
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationClemson University
cg.contributor.affiliationAgricultural Research Service
cg.contributor.affiliationNational Root Crops Research Institute, Nigeria
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryBenin
cg.coverage.countryCote D'Ivoire
cg.coverage.countryGhana
cg.coverage.countryNigeria
cg.coverage.countryTogo
cg.creator.identifierRanjana Bhattacharjee: 0000-0002-5184-5930
cg.creator.identifierPaterne AGRE: 0000-0003-1231-2530
cg.creator.identifierAntonio Jose Lopez-Montes: 0000-0001-5801-2475
cg.creator.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.creator.identifierRanajit Bandyopadhyay: 0000-0003-2422-4298
cg.researchthemeBIOTECH & PLANT BREEDING
cg.researchthemePLANT PRODUCTION & HEALTH
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectYam
cg.journalPLOS ONE
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid101146
cg.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0197717


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