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Prevalence and genome characterization of field isolates of sugarcane mosaic virus (SCMV) in Nigeria

dc.contributor.authorYayaya, A.
dc.contributor.authorDangora, D.B.
dc.contributor.authorKumar, P.L.
dc.contributor.authorAlegbejo, M.D.
dc.contributor.authorGregg, L.
dc.contributor.authorAlabi, O.J.
dc.date.accessioned2019-12-04T11:26:33Z
dc.date.available2019-12-04T11:26:33Z
dc.date.issued2019-05
dc.identifier.citationYahaya, A., Dangora, D.B., Kumar, P.L., Alegbejo, M., Gregg, L. & Alabi, O.J. (2019). Prevalence and genome characterization of field isolates of sugarcane mosaic virus (SCMV) in Nigeria. Plant Disease 103(5), 818-824.
dc.identifier.issn0191-2917
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4651
dc.description.abstractMaize and sugarcane are two economically important crops often grown in adjacent fields or co-cultivated in the northern guinea savannah agroecological zone, a major cereal production region of Nigeria. This study was conducted to determine the prevalence of mosaic disease in sugarcane and maize crops in the northern guinea savannah agroecological zone and to molecularly characterize the associated sugarcane mosaic virus (SCMV, genus Potyvirus) isolates. Surveys were conducted from June to July 2015 and sugarcane mosaic disease (SCMD) incidence was assessed across 21 farmer’s fields. Mean SCMD incidence varied across states with ~82% (308/376), ~66% (143/218) and ~67% (36/54) recorded in Kaduna, Kano and Katsina states, respectively. RT-PCR analysis of 415 field-collected samples using genus-specific primers confirmed potyvirus infection in 63.7% (156/245) of sugarcane, 29.7% (42/141) of maize crops and 45% (13/29) itch grass samples. Cloning and sequencing of gene-specific DNA amplicons from a subset of 45 samples (sugarcane = 33, maize = 9, itch grass = 3) confirmed their specificities to SCMV. Phylogenetic analysis of the partial gene sequences showed that they all belong to a single monophyletic clade of SCMV. These results were supported by analysis of complete polyprotein sequences of representative maize and sugarcane isolates from Nigeria. Both isolates shared 94.9%/97.3% complete polyprotein nucleotide (nt)/amino acid (aa) identities with each other and 75.2%/97.6% nt/aa identities with corresponding sequences of global SCMV isolates. The detection of identical population of SCMV isolates in both crop species and a weed host suggests possible vector mediated interspecies spread within cereal landscapes in the study area with implications for the integrated and sustainable management of SCMD in cereal cropping systems in Nigeria.
dc.format.extent1-33
dc.language.isoen
dc.rightsOther
dc.subjectPotyviruses
dc.subjectCereal
dc.subjectSugarcane Mosaic Virus
dc.subjectEpidemiology
dc.titlePrevalence and genome characterization of field isolates of sugarcane mosaic virus (SCMV) in Nigeria
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationAhmadu Bello University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationTexas A&M University
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.creator.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.researchthemePLANT PRODUCTION & HEALTH
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.journalPlant Disease
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid101413
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1094/PDIS-08-18-1445-RE


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