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dc.contributor.authorPellegrineschi, A.
dc.contributor.authorFatokun, C.A.
dc.contributor.authorThottappilly, G.
dc.contributor.authorAdepoju, A.A.
dc.identifier.citationPellegrineschi, A., Fatokun, C.A., Thottappilly, G. & Adepoju, A.A. (1997). Cowpea embryo rescue. 1. Influence of culture media composition on plant recovery from isolated immature embryos. Plant Cell Reports, 17(2), 133-138.
dc.description.abstractFactors responsible for successful rescue of immature embryos of cowpea [Vigna unguiculata (L.) Walp.] and V. vexillata (L.) and for in vitro embryo development were studied. A new basal medium for embryo development in vitro was formulated on the basis of the mineral composition of embryos. Sucrose, fructose and glucose were compared as carbohydrate sources. The highest frequency of embryos developing into plants was obtained with sucrose. Adding casein hydrolysate to the medium increased plant recovery by 30%. Among the plant growth factors used, cytokinins, zeatin, 6-benzylaminopurine and kinetin were the most effective in promoting embryo maturation and development. A method that can routinely ensure high plant recovery from cultured immature cowpea embryos is proposed.
dc.description.sponsorshipGovernment of Italy
dc.subjectVigna Unguiculata
dc.subjectMineral Salts
dc.titleCowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionWest Africa
cg.isijournalISI Journal
cg.authorship.typesCGIAR single centre
cg.iitasubjectPlant Breeding
cg.accessibilitystatusLimited Access

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