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    Identification of a defective molecule derived from DNAA of the bipartite begomovirus of East African Cassava Mosaic Virus

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    Date
    2006
    Author
    Ndunguru, J.
    Legg, J.
    Fofana, B.
    Aveling, T.
    Thompson, G.
    Fauquet, C.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    Geminivirus defective interfering DNAs arise spontaneously in mechanically inoculated test plants, and have previously been found with DNA‐B of the bipartite cassava mosaic geminiviruses, but not DNA‐A. Reported here for the first time is the cloning and characterization of a naturally occurring truncated form of cassava mosaic geminivirus DNA‐A, which at 1525 nt is around half the expected full size. Sequence analysis has shown it to be a defective (df) form of East African cassava mosaic virus (EACMV) DNA‐A that has retained its cis elements essential for replication by the helper virus, and it has been termed df DNA‐A 15. Phylogenetic comparisons placed the df DNA‐A 15 molecule close to mild and severe isolates of EACMV‐UG2. Biolistic inoculation of Nicotiana benthamiana with infectious df DNA‐A 15 clone and East African cassava mosaic Cameroon virus (EACMCV) resulted in symptom amelioration as compared with EACMCV singly inoculated plants, and there was an accumulation of df DNA‐A 15 in systemically infected leaves. In addition, the level of EACMV DNA‐B accumulation was reduced in the coinoculated plants compared with those inoculated with EACMCV alone. PCR and sequence analysis confirmed the helper virus as EACMV.
    https://doi.org/10.1111/j.1365-3059.2005.01289.x
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5272
    Digital Object Identifier (DOI)
    https://doi.org/10.1111/j.1365-3059.2005.01289.x
    IITA Subjects
    Plant Diseases; Pests Of Plants; Plant Genetic Resources; Plant Breeding; Cassava
    Agrovoc Terms
    Cassava Mosaic Virus; African Cassava Mosaic Virus; Cassava
    Regions
    Africa; Acp; Southern Africa; East Africa; North America; Europe
    Countries
    South Africa; Uganda; United States; United Kingdom
    Collections
    • Journal and Journal Articles4469
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