| dc.contributor.author | Bosque-Pérez, N.A. |
| dc.contributor.author | May, G. |
| dc.contributor.author | Artzen, C. |
| dc.date.accessioned | 2019-12-04T11:30:34Z |
| dc.date.available | 2019-12-04T11:30:34Z |
| dc.date.issued | 2000 |
| dc.identifier.citation | Bosque-Perez, N., May, G. & Artzen, C. (2000). Applicability of an Agribacterium-based system for the transformation of Musa species with diverse genomic constitution of ploidy level. Acta Horticulturae, 540, 193-210. |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/5326 |
| dc.description.abstract | An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains. |
| dc.language.iso | en |
| dc.subject | Genomics |
| dc.subject | Bananas |
| dc.subject | Clones |
| dc.subject | Musa |
| dc.subject | Agrobacterium |
| dc.title | Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.affiliation | Bioversity International |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.contributor.affiliation | Agriculture Division of DowDuPont |
| cg.contributor.affiliation | University of Arizona |
| cg.coverage.region | Africa |
| cg.coverage.region | Acp |
| cg.coverage.region | West Africa |
| cg.coverage.region | North America |
| cg.coverage.country | Nigeria |
| cg.coverage.country | United States |
| cg.authorship.types | CGIAR and advanced research institute |
| cg.iitasubject | Banana |
| cg.iitasubject | Food Security |
| cg.iitasubject | Genetic Improvement |
| cg.iitasubject | Plant Breeding |
| cg.iitasubject | Plant Diseases |
| cg.accessibilitystatus | Limited Access |
| local.dspaceid | 103683 |
| cg.identifier.doi | https://doi.org/10.17660/ActaHortic.2000.540.21 |
