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dc.contributor.authorBosque-Pérez, N.A.
dc.contributor.authorMay, G.
dc.contributor.authorArtzen, C.
dc.date.accessioned2019-12-04T11:30:34Z
dc.date.available2019-12-04T11:30:34Z
dc.date.issued2000
dc.identifier.citationBosque-Perez, N., May, G. & Artzen, C. (2000). Applicability of an Agribacterium-based system for the transformation of Musa species with diverse genomic constitution of ploidy level. Acta Horticulturae, 540, 193-210.
dc.identifier.urihttps://hdl.handle.net/20.500.12478/5326
dc.description.abstractAn Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains.
dc.language.isoen
dc.subjectGenomics
dc.subjectBananas
dc.subjectClones
dc.subjectMusa
dc.subjectAgrobacterium
dc.titleApplicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationBioversity International
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationAgriculture Division of DowDuPont
cg.contributor.affiliationUniversity of Arizona
cg.coverage.regionAfrica
cg.coverage.regionAcp
cg.coverage.regionWest Africa
cg.coverage.regionNorth America
cg.coverage.countryNigeria
cg.coverage.countryUnited States
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectBanana
cg.iitasubjectFood Security
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.accessibilitystatusLimited Access
local.dspaceid103683
cg.identifier.doihttps://doi.org/10.17660/ActaHortic.2000.540.21


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