• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Wet-cold preservation of West African yam (Dioscorea spp.) pollen

    Thumbnail
    Date
    2002
    Author
    Daniel, I.O.
    Ng, N.Q.
    Tayo, T.O.
    Togun, A.O.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    Hermetic cold storage without previous drying (wet–cold storage) was experimented for the preservation of yam pollen grains. Pollens collected from white yam D. rotundata and a wild relative, D. praehensilis, were stored at -80, -20, 5, and 150C in sealed packs for 2 years. Pollen samples were drawn for in vitro germination tests after 100 and 700 days, and hand pollination was conducted after 365 and 730 days in storage. Pollen germination responses were not significantly different among the two species. Though pollen maintained germination capacity at all the storage temperature regimes, there was a significant loss in the viability of pollen stored at 5 and 15 _C after 100 days of storage. After 700 days in storage, pollens stored at 5 and 150C had lost germination capacity while there were no significant differences in the germination of fresh pollen and pollen frozen at -80 and -200C. Hand pollination with pollen of D. rotundata frozen at -800C for 365 days gave 69.5% fruit set and 50% fruit set after 730 days in storage. From these results, the wet–freeze procedure appears promising to execute the establishment of pollen gene banks for yam breeding and for conservation of haploid gene pool of yams in base collections. A pollen storage protocol based on the procedure is recommended.
    https://doi.org/10.1017_S0021859601001708
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5396
    Digital Object Identifier (DOI)
    https://doi.org/10.1017_S0021859601001708
    IITA Subjects
    Genetic Improvement; Yam
    Agrovoc Terms
    Yams; Pollination; Germination; Pollen; Haploidy; Gene Banks; Gene Pools
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository