• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Books and Book Chapters
    • Books and Book Chapters
    • View Item
    •   Home
    • Books and Book Chapters
    • Books and Book Chapters
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Development of micropropagation system for yam (Dioscorea spp.) using somatic embryogenesis

    Thumbnail
    View/Open
    U18BkChukwunaluImprovingNothomNodev.pdf (1.830Mb)
    Date
    2018
    Author
    Ossai, C.
    Balogun, M.
    Maroya, N.
    Asiedu, Robert
    Type
    Book
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Inadequate availability of disease-free planting materials remains a major constraint to yam production. The tissue culture technique has been used to regenerate disease-free plantlets from pre-formed, heattreated meristems followed by micropropagation. This procedure, however, has a low multiplication ratio with an average of 1: 4 every eight weeks. Embryo production from somatic cells (somatic embryogenesis, SE) is a system in which each somatic cell can regenerate a complete plantlet. However, previous reports show low SE induction frequencies and significant variations in success rates among different genotypes while hardly any report exist for improved varieties that farmers desire, especially in Nigeria. Studies were carried out to evaluate the effects of different plant growth regulators (PGRs) on induction of somatic embryogenesis of the following genotypes: one improved Dioscorea alata (TDa 291) and three improved (TDr 95/19177, TDr 89/2665, TDr 95/18544) and one landrace (Obioturugo) of Dioscorea rotundata. Leaf, stem, and axillary bud explants were cultured in MS basal medium containing fifteen treatment combinations of 2,4-Dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA), Benzylaminopurine (BAP), Picloram, and Uniconazole-P (UP). The genotype TDr 95/19177 was tested for SE in Temorary Immersion Bioreactor System (TIBS). The incidence of induction of callus formation and plantlet regeneration from the three explants were recorded. Embryogenic callus induction was highest (87%) from axillary buds cultured on modified MS + 2 mg/l of 2,4-D + 1 mg/l of NAA while 1 mg/l of BAP + 9. 9 mg/l of UP had the highest percentage plantlet regeneration of 50% in TDr 95/18544 and an average of 37% across genotypes at a mean of 5 plantlets per explant. The genotype TDr 95/19177 was successfully regenerated via indirect somatic embryogenesis in the SETIS Type Temporary Immersion Bioreactor System.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5521
    Non-IITA Authors ORCID
    Morufat Balogunhttps://orcid.org/0000-0001-8770-5529
    Norbert Maroyahttps://orcid.org/0000-0002-7079-4729
    Robert Asieduhttps://orcid.org/0000-0001-8943-2376
    Research Themes
    BIOTECH & PLANT BREEDING
    IITA Subjects
    Food Science; Food Security; Plant Breeding; Yam
    Agrovoc Terms
    Yams; Food Security; Food Production; West Africa; Nigeria; Somatic Embryos; Callus; Bioreactors
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Books and Book Chapters951
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository