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Development of micropropagation system for yam (Dioscorea spp.) using somatic embryogenesis

dc.contributor.authorOssai, C.
dc.contributor.authorBalogun, M.
dc.contributor.authorMaroya, N.
dc.contributor.authorAsiedu, Robert
dc.date.accessioned2019-12-04T11:31:32Z
dc.date.available2019-12-04T11:31:32Z
dc.date.issued2018
dc.identifier.citationOssai, C., Balogun, M., Maroya, N. & Asiedu, R. (2018). Development of micropropagation system for yam (Dioscorea spp.) using somatic embryogenesis. Ibadan, Nigeria: IITA, (8p).
dc.identifier.isbn978-978-131-356-1
dc.identifier.urihttps://hdl.handle.net/20.500.12478/5521
dc.description.abstractInadequate availability of disease-free planting materials remains a major constraint to yam production. The tissue culture technique has been used to regenerate disease-free plantlets from pre-formed, heattreated meristems followed by micropropagation. This procedure, however, has a low multiplication ratio with an average of 1: 4 every eight weeks. Embryo production from somatic cells (somatic embryogenesis, SE) is a system in which each somatic cell can regenerate a complete plantlet. However, previous reports show low SE induction frequencies and significant variations in success rates among different genotypes while hardly any report exist for improved varieties that farmers desire, especially in Nigeria. Studies were carried out to evaluate the effects of different plant growth regulators (PGRs) on induction of somatic embryogenesis of the following genotypes: one improved Dioscorea alata (TDa 291) and three improved (TDr 95/19177, TDr 89/2665, TDr 95/18544) and one landrace (Obioturugo) of Dioscorea rotundata. Leaf, stem, and axillary bud explants were cultured in MS basal medium containing fifteen treatment combinations of 2,4-Dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA), Benzylaminopurine (BAP), Picloram, and Uniconazole-P (UP). The genotype TDr 95/19177 was tested for SE in Temorary Immersion Bioreactor System (TIBS). The incidence of induction of callus formation and plantlet regeneration from the three explants were recorded. Embryogenic callus induction was highest (87%) from axillary buds cultured on modified MS + 2 mg/l of 2,4-D + 1 mg/l of NAA while 1 mg/l of BAP + 9. 9 mg/l of UP had the highest percentage plantlet regeneration of 50% in TDr 95/18544 and an average of 37% across genotypes at a mean of 5 plantlets per explant. The genotype TDr 95/19177 was successfully regenerated via indirect somatic embryogenesis in the SETIS Type Temporary Immersion Bioreactor System.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent8 p.
dc.language.isoen
dc.publisherInternational Institute of Tropical Agriculture
dc.relation.ispartofseriesYIIFSWA Research Brief: Improving Yam Micropropagation Series 2;
dc.rightsCC-BY-4.0
dc.subjectYams
dc.subjectFood Security
dc.subjectFood Production
dc.subjectWest Africa
dc.subjectNigeria
dc.subjectSomatic Embryos
dc.subjectCallus
dc.subjectBioreactors
dc.titleDevelopment of micropropagation system for yam (Dioscorea spp.) using somatic embryogenesis
dc.typeBook
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Ibadan
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.creator.identifierMorufat Balogun: 0000-0001-8770-5529
cg.creator.identifierNorbert Maroya: 0000-0002-7079-4729
cg.creator.identifierRobert Asiedu: 0000-0001-8943-2376
cg.researchthemeBIOTECH & PLANT BREEDING
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectFood Science
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectYam
cg.howpublishedFormally Published
cg.publicationplaceIbadan, Nigeria
cg.accessibilitystatusOpen Access
local.dspaceid104019
cg.targetaudienceScientists


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