Show simple item record

Transient gene expression in electroporated banana (Musa spp., cv. Bluggoe, ABB group) protoplasts isolated from regenerable embryogenic cell suspensions

dc.contributor.authorSagi, L.
dc.contributor.authorRemy, S.
dc.contributor.authorPanis, B.
dc.contributor.authorSwennen, R.
dc.contributor.authorVolckaert, G.
dc.date.accessioned2019-12-04T11:33:17Z
dc.date.available2019-12-04T11:33:17Z
dc.date.issued1994
dc.identifier.citationSagi, L., Remy, S., Panis, B., Swennen, R. & Volckaert, G. (1994). Transient gene expression in electroporated banana (Musa spp., cv.'Bluggoe', ABB group) protoplasts isolated from regenerable embryogenetic cell suspensions. Plant Cell Reports, 13(5), 262-266.
dc.identifier.issn0721-7714
dc.identifier.urihttps://hdl.handle.net/20.500.12478/5605
dc.description.abstractElectroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol treatment and its duration before electroporation, use of a heat shock, and chimaeric gene constructs. The maximum frequency of DNA introduction as detected by an in situ assay for transient expression of the uidA gene, amounted to 1.8% of total protoplasts. Since plants have recently been regenerated from banana protoplasts at a high frequency, the present results may contribute to the production of transgenic banana.
dc.language.isoen
dc.subjectBananas
dc.subjectDna
dc.subjectGenes
dc.titleTransient gene expression in electroporated banana (Musa spp., cv. Bluggoe, ABB group) protoplasts isolated from regenerable embryogenic cell suspensions
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationKatholieke Universiteit, Leuven
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionEurope
cg.coverage.regionWest Africa
cg.coverage.countryBelgium
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPlant Breeding
cg.iitasubjectBanana
cg.iitasubjectFood Security
cg.accessibilitystatusLimited Access
local.dspaceid104885


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record