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    Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions

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    Date
    1995
    Author
    Sagi, L.
    Remy, S.
    Verelst, B.
    Panis, B.
    Cammue, B.P.
    Volckaert, G.
    Swennen, R.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    In order to introduce currently-available genes with agronomical value into banana, two genetic transformation protocols have been optimized. Firstly, regenerable protoplasts isolated from embryogenic cell suspensions of the cultivar Bluggoe have been used for the introduction of several chimaeric uidA gene constructs by electroporation. With the inclusion of polyethylene glycol and heat shock, the frequency of transiently expressing protoplasts reached 1.8% as shown by an in situ β-glucuronidase assay. A duplicated 35S promoter with an alfalfa mosaic virus leader sequence (pBI-426) induced the highest expression rate among the constructs tested. Embryogenic cell suspensions of cv. Bluggoe have also been bombarded with accelerated particles coated with a high expression uidA gene construct (pEmuGN) using a biolistic gun. After a partial optimization of the procedure, transient GUS assays reproducibly demonstrated the presence of 400 blue foci in 30 μl of settled cell volume (approximately 25 mg cells). Selection and characterization of antibiotic-resistant transformed cultures is in progress.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5772
    IITA Subjects
    Plant Genetic Resources; Plant Breeding; Banana
    Agrovoc Terms
    Genetics; Bananas; Protoplasts; Embryos (Plant)
    Regions
    Acp; Africa; Europe; West Africa
    Countries
    Belgium; Nigeria
    Collections
    • Journal and Journal Articles4836
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