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Influence of reference trees on N2fixation estimates in Leucaena leucocephala and Acacia albicia using 15N_ labelling techniques+A51

dc.contributor.authorSanginga, N.
dc.contributor.authorDanso, S.K.A.
dc.contributor.authorZapata, F.
dc.contributor.authorBowen, G.D.
dc.date.accessioned2019-12-04T11:34:35Z
dc.date.available2019-12-04T11:34:35Z
dc.date.issued1990
dc.identifier.citationSanginga, N., Danso, S.K.A., Zapata, F. & Bowen, G.D. (1990). Influence of reference trees on N 2-fixation estimates in Leucaena leucocephala and Acacia albida using 15 N-labelling techniques. Biology and Fertility of Soils, 9(4), 341-346.
dc.identifier.issn1432-0789
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6019
dc.description.abstractWe examined the suitability of four reference crops, i.e., two non-fixing trees,Cassia siamea andEucalyptus grandis, and two uninoculated fixing trees,Leucaena leucocephala andAcacia albida, for measuring fixed N2 fixed in inoculatedL. leucocephala andA. albida grown for 36 weeks in pots. The15N isotope-dilution (involving the addition of equal amounts of labelled N fertilizer to the non-fixing and the fixing plants) and theA-value (with different amounts of labelled N fertilizer added to the fixing and the non-fixing crops) methods were used. The isotope dilution approach gave several large negative estimates of fixed N2 inA. albida. Positive and similar values of fixed N2 were measured in all four reference crops using theA-value approach. ForL. leucocephala the isotope-dilution approach gave different estimates of fixed N2, with the different reference crops; the uninoculated N2-fixing crops indicated significantly less fixed N2 than the non-fixing reference crops. Similar values for N2 fixed inL. leucocephala were obtained using the two non-fixing trees, either by the isotope-dilution or theA-value method. On average,A. albida derived about twice as much N from fertilizer asL. leucocephala. In both species, the atom %15N excess declined by about 50% in successive harvests.
dc.language.isoen
dc.subjectAcacia Albida
dc.subjectCassia Siamea
dc.subjectEucalyptus Grandis
dc.subjectNitrogen Fixing Trees
dc.subjectForest Rehabilitation
dc.subjectIsotope Dilution Method
dc.subjectLeucaena Leucocephala
dc.subjectRhizobium
dc.titleInfluence of reference trees on N2fixation estimates in Leucaena leucocephala and Acacia albicia using 15N_ labelling techniques+A51
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationInternational Atomic Energy Agency
cg.coverage.regionAcp
cg.coverage.regionEurope
cg.coverage.countryAustria
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectGrain Legumes
cg.iitasubjectSoil Fertility
cg.iitasubjectPlant Diseases
cg.accessibilitystatusLimited Access
local.dspaceid105949
cg.identifier.doihttps://doi.org/10.1007/BF00634112


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