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    Cryopreservation of cassava (Manihot esculenta Crantz)

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    Date
    2004
    Author
    Adeniyi, O.J.
    Akoroda, M.O.
    Ng, S.Y.
    Type
    Conference Paper
    Review Status
    Peer Review
    Metadata
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    Abstract/Description
    This study intended to develop and/or adopt the best cryopresentation protocol for the African cassava germplasm. Eleven protocols were investigated. One protocol focused on the tolerance of pregrowth meristems to DMSO (Dimethyl sulphoxide). The other 10 were cryopresentation protocols and were employed to determine the best possible procedure involved in terms of recovery rate. Seven cultivars obtained from the International Institute of Tropical Agriculture were used. These include TMS M80/00106, TMS 63397,TMS 60142, TMS 30001, TMS 91/02324, TME 1 and TME2. Amongest the seven DMSO concentrations tested on pregrowth meristems of four genotypes, 0.5% (v/v) DMSO was the right concentration in which highest telerance was found. From the ten cryopresentation protocols carried out, five different dehydration period were tested. A 5-hr dehydration, using alginate-bead dehydration method of cryopresentation emerged the best. It was evident that modification introduced at the cryopresentation stage was reasonable as 5% DMSO performed best when used alone as a cryopretectant while 0.5% DMSO combined with calcium alginate and high sucrose proved equally good for cryopreservation of meristems and node cutting.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/6272
    IITA Subjects
    Cassava; Knowledge Management; Handling, Transport, Storage And Protection Of Agricultural Products; Plant Production
    Agrovoc Terms
    Cassava; Cryopreservation; Manihot Esculenta; Genotypes
    Regions
    Africa; West Africa
    Countries
    Nigeria
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