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dc.contributor.authorAdeniyi, O.J.
dc.contributor.authorAkoroda, M.O.
dc.contributor.authorNg, S.Y.
dc.date.accessioned2019-12-04T11:36:31Z
dc.date.available2019-12-04T11:36:31Z
dc.date.issued2004
dc.identifier.citationAdeniyi, O.J., Akoroda, M.O. & Ng, S.Y. (2004). Cryopreservation of cassava (Manihot esculenta Crantz). In The small processor and development of local food industries for market economy: proceedings of the Eighth Symposium of the International Society for Tropical Root Crops - Africa Branch (ISTRC-AB), 12-16 November 2001, IITA, Ibadan, Nigeria. Ibadan, Nigeria: IITA, (p. 502-505).
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6272
dc.description.abstractThis study intended to develop and/or adopt the best cryopresentation protocol for the African cassava germplasm. Eleven protocols were investigated. One protocol focused on the tolerance of pregrowth meristems to DMSO (Dimethyl sulphoxide). The other 10 were cryopresentation protocols and were employed to determine the best possible procedure involved in terms of recovery rate. Seven cultivars obtained from the International Institute of Tropical Agriculture were used. These include TMS M80/00106, TMS 63397,TMS 60142, TMS 30001, TMS 91/02324, TME 1 and TME2. Amongest the seven DMSO concentrations tested on pregrowth meristems of four genotypes, 0.5% (v/v) DMSO was the right concentration in which highest telerance was found. From the ten cryopresentation protocols carried out, five different dehydration period were tested. A 5-hr dehydration, using alginate-bead dehydration method of cryopresentation emerged the best. It was evident that modification introduced at the cryopresentation stage was reasonable as 5% DMSO performed best when used alone as a cryopretectant while 0.5% DMSO combined with calcium alginate and high sucrose proved equally good for cryopreservation of meristems and node cutting.
dc.language.isoen
dc.subjectCassava
dc.subjectCryopreservation
dc.subjectManihot Esculenta
dc.subjectGenotypes
dc.titleCryopreservation of cassava (Manihot esculenta Crantz)
dc.typeConference Paper
cg.contributor.affiliationUniversity of Ibadan
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCassava
cg.iitasubjectKnowledge Management
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectPlant Production
cg.accessibilitystatusLimited Access
cg.reviewstatusPeer Review
local.dspaceid107554


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