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dc.contributor.authorNakato, G.V.
dc.contributor.authorRojas, J.L.F.
dc.contributor.authorVerniere, C.
dc.contributor.authorBlondin, L.
dc.contributor.authorCoutinho, T.
dc.contributor.authorMahuku, G.
dc.contributor.authorWicker, E.
dc.date.accessioned2019-12-04T11:36:46Z
dc.date.available2019-12-04T11:36:46Z
dc.date.issued2019-04-11
dc.identifier.citationNakato, G.V., Rojas, J.L.F., Verniere, C., Blondin, L., Coutinho, T., Mahuku, G. & Wicker, E. (2019). A new multi locus variable number of tandem repeat analysis scheme for epidemiological surveillance of Xanthomonas vasicola pv. musacearum, the plant pathogen causing bacterial wilt on banana and enset. PLOS ONE, 14(4): e0215090, 1-22.
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6344
dc.descriptionOpen Access Journal
dc.description.abstractXanthomonas vasicola pv. musacearum (Xvm) which causes Xanthomonas wilt (XW) on banana (Musa accuminata x balbisiana) and enset (Ensete ventricosum), is closely related to the species Xanthomonas vasicola that contains the pathovars vasculorum (Xvv) and holcicola (Xvh), respectively pathogenic to sugarcane and sorghum. Xvm is considered a monomorphic bacterium whose intra-pathovar diversity remains poorly understood. With the sudden emergence of Xvm within east and central Africa coupled with the unknown origin of one of the two sublineages suggested for Xvm, attention has shifted to adapting technologies that focus on identifying the origin and distribution of the genetic diversity within this pathogen. Although microbiological and conventional molecular diagnostics have been useful in pathogen identification. Recent advances have ushered in an era of genomic epidemiology that aids in characterizing monomorphic pathogens. To unravel the origin and pathways of the recent emergence of XW in Eastern and Central Africa, there was a need for a genotyping tool adapted for molecular epidemiology. Multi-Locus Variable Number of Tandem Repeat Analysis (MLVA) is able to resolve the evolutionary patterns and invasion routes of a pathogen. In this study, we identified microsatellite loci from nine published Xvm genome sequences. Of the 36 detected microsatellite loci, 21 were selected for primer design and 19 determined to be highly typeable, specific, reproducible and polymorphic with two- to four- alleles per locus on a sub-collection. The 19 markers were multiplexed and applied to genotype 335 Xvm strains isolated from seven countries over several years. The microsatellite markers grouped the Xvm collection into three clusters; with two similar to the SNP-based sublineages 1 and 2 and a new cluster 3, revealing an unknown diversity in Ethiopia. Five of the 19 markers had alleles present in both Xvm and Xanthomonas vasicola pathovars holcicola and vasculorum, supporting the phylogenetic closeliness of these three pathovars. Thank to the public availability of the haplotypes on the MLVABank database, this highly reliable and polymorphic genotyping tool can be further used in a transnational surveillance network to monitor the spread and evolution of XW throughout Africa.. It will inform and guide management of Xvm both in banana-based and enset-based cropping systems. Due to the suitability of MLVA-19 markers for population genetic analyses, this genotyping tool will also be used in future microevolution studies.
dc.format.extent1-22
dc.language.isoen
dc.rightsCC-BY-4.0
dc.subjectXanthomonas Vasicola Pv. Musacearum
dc.subjectPlant Diseases
dc.subjectFood Security
dc.subjectCentral Africa
dc.subjectEast Africa
dc.subjectBananas
dc.subjectEnset
dc.titleA new Multi Locus Variable Number of Tandem Repeat Analysis Scheme for epidemiological surveillance of Xanthomonas vasicola pv. musacearum, the plant pathogen causing bacterial wilt on banana and enset
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Montpellier
cg.contributor.affiliationUnité Mixte de Recherche - Biologie et Génétique des Interactions Plante-Parasite
cg.contributor.affiliationUniversity of Pretoria
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.regionEast Africa
cg.coverage.countryCongo, Dr
cg.coverage.countryEthiopia
cg.coverage.countryKenya
cg.coverage.countryRwanda
cg.coverage.countryTanzania
cg.creator.identifierGeorge Mahuku: 0000-0001-8444-8651
cg.researchthemePLANT PRODUCTION & HEALTH
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.journalPLOS ONE
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid107734
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1371/journal. pone.0215090


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