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Attempts to identify Cassava Brown Streak Virus in western Democratic Republic of Congo

dc.contributor.authorBakelana, Z.
dc.contributor.authorMagembe, E.
dc.contributor.authorLaura, M.B.
dc.contributor.authorMacharia, M.
dc.contributor.authorMahungu, N.
dc.contributor.authorTata-Hangy, W.
dc.contributor.authorLutete, D.
dc.contributor.authorMonde, G.
dc.contributor.authorHarvey, J.
dc.contributor.authorNdunguru, J.
dc.contributor.authorKayuki, C.
dc.contributor.authorPita, J.
dc.contributor.authorLema, M.
dc.contributor.authorKanana, T.
dc.date.accessioned2020-02-27T10:15:43Z
dc.date.available2020-02-27T10:15:43Z
dc.date.issued2019-01-15
dc.identifier.citationBakelana, Z., Magembe, E., Laura, M.B., Macharia, M., Mahungu, N., Tata-Hangy, W., ... & Kanana, T. (2019). Attempts to identify Cassava Brown Streak Virus in western Democratic Republic of Congo. Journal of Agricultural Science. 11(2), 31-39.
dc.identifier.issn1916-9752
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6796
dc.description.abstractRoot necrosis similar to those of the cassava brown streak disease (CBSD) were observed on cassava in western provinces of the Democratic Republic of Congo (DR.Congo) in the early 2000’s. However molecular laboratory diagnosis were not able to detect any causative agent responsible for the attacks, hence, the disease related to these symptoms was named CBSD-like disease. In order to assess the distribution and the incidence of the CBSD-like disease, surveys were carried out in four western provinces, comprising, Kwango and Kwilu, Sud Ubangi, Kinshasa and Kongo Central. CBSD-like disease was observed in all surveyed provinces on the basis of root symptoms because foliar symptoms were different to those of the documented cases of CBSD in other parts of east Africa. CBSD-like disease incidence was high in Kongo Central and Sud Ubangi, exceeding an average of 50 %, but low in Kwango and Kwilu (32.8%) and in Kinshasa (19.1%). During the surveys, cassava leaf samples were collected for lab identification of the causal agent. PCR diagnosis was done on these samples using primers specific for the two known CBSVs. All samples tested negative with no amplification of DNA fragments of the correct size. Thus, further analysis on the causative organism is needed using Next Generation Sequencing (NGS) approaches. NGS approaches will help also to identify the causative organism in other Central Africa countries (Angola, Congo-Brazzaville and Gabon) where such cassava root necrosis have been reported or are suspected.
dc.description.sponsorshipINERA National Cassava Program
dc.description.sponsorshipAfrica Biosciences Challenge Fund
dc.description.sponsorshipFood and Agriculture Organization of the United Nations
dc.format.extent31-39
dc.language.isoen
dc.subjectCassava
dc.subjectDemocratic Republic of the Congo
dc.subjectSurveys
dc.subjectNecrosis
dc.subjectRoots
dc.titleAttempts to identify Cassava Brown Streak Virus in western Democratic Republic of Congo
dc.typeJournal Article
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationNational Institute for Agricultural Research and Studies, DR. Congo
cg.contributor.affiliationInternational Livestock Research Institute
cg.contributor.affiliationUniversity of Western Australia
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationFood and Agriculture Organization of the United Nations
cg.contributor.affiliationInstitute Faculty of Agricultural Sciences, Yangambi
cg.contributor.affiliationMikocheni Agriculture Research Institute
cg.contributor.affiliationWest African Virus Epidemiology
cg.contributor.affiliationKinshasa University
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.countryDemocratic Republic of the Congo
cg.coverage.hubCentral Africa Hub
cg.creator.identifierNzola Mahungu: 0000-0002-3781-0838
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidBAKELANA:2019
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectCassava
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.journalJournal of Agricultural Science
cg.notesOpen Access Journal; Published online: Jan 15 2020
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.5539/jas.v11n2p31


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