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Detection and diversity of maize yellow mosaic virus infecting maize in Nigeria

dc.contributor.authorYahaya, A.
dc.contributor.authorDangora, D.B.
dc.contributor.authorAlabi, O.J.
dc.contributor.authorZongoma, A.M.
dc.contributor.authorKumar, P.L.
dc.date.accessioned2020-03-04T07:51:45Z
dc.date.available2020-03-04T07:51:45Z
dc.date.issued2019-12
dc.identifier.citationYahaya, A., Dangora, D.B., Alabi, O.J., Zongoma, A.M. & Kumar, P.L. (2019). Detection and diversity of maize yellow mosaic virus infecting maize in Nigeria. VirusDisease, 30(4), 538-544.
dc.identifier.issn2347-3584
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6807
dc.description.abstractMaize yellow mosaic virus (MaYMV; genus Polerovirus; family Luteoviridae) was recently characterized from maize in China and subsequently detected in mixed infection with sugarcane mosaic virus (genus Potyvirus; family Potyviridae) in sugarcane and itch grass in Nigeria. This study was conducted to understand the status and genetic diversity of MaYMV in maize fields in the northern guinea savannah region of Nigeria. A survey was conducted in 2017 and maize (n = 90) and itch grass (n = 10) plants suspected of virus infection based on appearance of mosaic and/or yellowing symptoms were sampled in Kaduna (n = 65) and Katsina (n = 35) states. The samples were screened individually by reverse transcription polymerase chain reaction using the genus-specific primers targeting poleroviruses and potyviruses Pol-G-F and Pol-G-R primers encompassing the partial P1–P2 fusion protein and coat protein genes of poleroviruses and primer pair CI-For & CI-Rev encompassing the partial cylindrical inclusion proteins of most potyviruses. A subset of amplified DNA fragments was cloned, Sanger-sequenced, and the obtained sequences were bioinformatically analyzed along with corresponding sequences from GenBank. The ~ 1.1 Kb polerovirus fragment was detected in 32.2% (29/90) of the maize samples while all 10 itch grass samples tested negative. BLASTN analysis of sequences derived from six polerovirus samples confirmed the virus identity as MaYMV. In pairwise comparisons, the MaYMV sequences derived in this study shared 97–99% nucleotide identity with sequences of other MaYMV isolates available in the NCBI GenBank. Phylogenetic analysis revealed the segregation of global MaYMV sequences into three host-independent clusters with pattern of geographic structuring.
dc.description.sponsorshipUnited States Agency for International Development
dc.description.sponsorshipBorlaug LEAP Program
dc.description.sponsorshipAhmadu Bello University
dc.description.sponsorshipTexas A&M AgriLife Research & Extension Center;
dc.description.sponsorshipCGIAR Research Program on Maize
dc.description.sponsorshipAmina Ado Foundation
dc.format.extent538-544
dc.language.isoen
dc.subjectLuteoviridae
dc.subjectPolerovirus
dc.subjectMaize
dc.subjectPhylogeny
dc.subjectNigeria
dc.subjectPlant Viruses
dc.titleDetection and diversity of maize yellow mosaic virus infecting maize in Nigeria
dc.typeJournal Article
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationAhmadu Bello University
cg.contributor.affiliationTexas A&M AgriLife Research and Extension Center
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.creator.identifierAdama Yahaya: 0000-0003-0520-8778
cg.creator.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.researchthemePlant Production and Health
cg.identifier.bibtexciteidYAHAYA:2019
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectDisease Control
cg.iitasubjectMaize
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.journalVirusDisease
cg.notesPublished online: 21 Nov 2019
cg.accessibilitystatusLimited Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCopyrighted; all rights reserved
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1007/s13337-019-00555-0


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