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Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield

dc.contributor.authorObata, T.
dc.contributor.authorKlemens, P.A.W.
dc.contributor.authorRosado-Souza, L.
dc.contributor.authorSchlereth, A.
dc.contributor.authorGisel, A.
dc.contributor.authorStavolone, L.
dc.contributor.authorZierer, W.
dc.contributor.authorMorales, N.
dc.contributor.authorMuller, L.A.
dc.contributor.authorZeeman, S.C.
dc.contributor.authorLudewig, F.
dc.contributor.authorStitt, M.
dc.contributor.authorSonnewald, U.
dc.contributor.authorNeuhaus, H.E.
dc.contributor.authorFernie, A.R.
dc.date.accessioned2020-05-28T12:33:33Z
dc.date.available2020-05-28T12:33:33Z
dc.date.issued2020
dc.identifier.citationObata, T., Klemens, P.A., Rosado‐Souza, L., Schlereth, A., Gisel, A., Stavolone, L., ... & Fernie, A.R. (2020). Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield. The Plant Journal, 1-40.
dc.identifier.issn0960-7412
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6849
dc.description.abstractCassava is an important staple crop in sub‐Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this high productivity are poorly understood including the mode of photosynthesis, reasons for the high rate of photosynthesis, the extent of source/sink limitation, the impact of environment, and the extent of variation between cultivars. Six commercial African cassava cultivars were grown in a greenhouse in Erlangen, Germany, and in the field in Ibadan, Nigeria. Source leaves, sink leaves, stems and storage roots were harvested during storage root bulking and analyzed for sugars, organic acids, amino acids, phosphorylated intermediates, minerals, starch, protein, activities of enzymes in central metabolism and yield traits. High ratios of RuBisCO:phosphoenolpyruvate carboxylase activity support a C3 mode of photosynthesis. The high rate of photosynthesis is likely to be attributed to high activities of enzymes in the Calvin–Benson cycle and pathways for sucrose and starch synthesis. Nevertheless, source limitation is indicated because root yield traits correlated with metabolic traits in leaves rather than in the stem or storage roots. This situation was especially so in greenhouse‐grown plants, where irradiance will have been low. In the field, plants produced more storage roots. This was associated with higher AGPase activity and lower sucrose in the roots, indicating that feedforward loops enhanced sink capacity in the high light and low nitrogen environment in the field. Overall, these results indicated that carbon assimilation rate, the K battery, root starch synthesis, trehalose, and chlorogenic acid accumulation are potential target traits for genetic improvement.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.description.sponsorshipUniversity of Nebraska‐Lincoln
dc.format.extent1-40
dc.language.isoen
dc.subjectCassava
dc.subjectYields
dc.subjectPhotosynthesis
dc.subjectEnzyme Activity
dc.subjectNitrogen Metabolism
dc.subjectStarch
dc.subjectChlorogenic Acid
dc.titleMetabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationMax‐Planck‐Institute of Molecular Plant Physiology
cg.contributor.affiliationUniversity of Kaiserslautern
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Erlangen‐Nuremberg
cg.contributor.affiliationBoyce Thompson Institute, USA
cg.contributor.affiliationInstitute of Molecular Plant Biology, Switzerland
cg.contributor.affiliationInstitute for Biomedical Technologies, Italy
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidOBATA:2020
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAgronomy
cg.iitasubjectCassava
cg.iitasubjectFood Security
cg.iitasubjectFood Systems
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectValue Chains
cg.journalThe Plant Journal
cg.notesOpen Access Article; Published online: 04 March 2020
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1111/tpj.14693
cg.iitaauthor.identifierANDREAS GISEL: 0000-0001-7218-9488
cg.iitaauthor.identifierLIVIA STAVOLONE: 0000-0002-0691-1302
cg.noniitaauthor.identifierLukas Muller: 0000-0001-8640-1750
cg.noniitaauthor.identifierUwe Sonnewald: 0000-0003-1835-5339
cg.noniitaauthor.identifierAlisdair Fernie: 0000-0001-9000-335X


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