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Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing

dc.contributor.authorShenge, K.C.
dc.contributor.authorAdhikari, B.N.
dc.contributor.authorAkande, A.
dc.contributor.authorCallicott, K.A.
dc.contributor.authorAtehnkeng, J.
dc.contributor.authorOrtega-Beltran, A.
dc.contributor.authorKumar, P.L.
dc.contributor.authorBandyopadhyay, R.
dc.contributor.authorCotty, P.J.
dc.date.accessioned2020-08-05T08:43:46Z
dc.date.available2020-08-05T08:43:46Z
dc.date.issued2019
dc.identifier.citationShenge, K.C., Adhikari, B.N., Akande, A., Callicott, K.A., Atehnkeng, J., Ortega-Beltran, A., ... & Cotty, P. J. (2019). Monitoring A. flavus with pyrosequencing monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing. Frontiers in Microbiology, 10, 1-13.
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6906
dc.description.abstractAflatoxins pose significant food security and public health risks, decrease productivity and profitability of animal industries, and hamper trade. To minimize aflatoxin contamination in several crops, a biocontrol technology based on atoxigenic strains of Aspergillus flavus is commercially used in the United States and some African countries. Significant efforts are underway to popularize the use of biocontrol in Africa by various means including incentives. The purpose of this study was to develop quantitative pyrosequencing assays for rapid, simultaneous quantification of proportions of four A. flavus biocontrol genotypes within complex populations of A. flavus associated with maize crops in Nigeria to facilitate payment of farmer incentives for Aflasafe (a biocontrol product) use. Protocols were developed to confirm use of Aflasafe by small scale farmers in Nigeria. Nested PCR amplifications followed by sequence by synthesis pyrosequencing assays were required to quantify frequencies of the active ingredients and, in so doing, confirm successful use of biocontrol by participating farmers. The entire verification process could be completed in 3–4 days proving a savings over other monitoring methods in both time and costs and providing data in a time frame that could work with the commercial agriculture scheme. Quantitative pyrosequencing assays represent a reliable tool for rapid detection, quantification, and monitoring of multiple A. flavus genotypes within complex fungal communities, satisfying the requirements of the regulatory community and crop end-users that wish to determine which purchased crops were treated with the biocontrol product. Techniques developed in the current study can be modified for monitoring other crop-associated fungi.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-13
dc.language.isoen
dc.subjectAflatoxins
dc.subjectBiological Control
dc.subjectMonitoring
dc.subjectMaize
dc.subjectAspergillus Flavus
dc.subjectGenotypes
dc.subjectNigeria
dc.titleMonitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing
dc.typeJournal Article
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUnited States Department of Agriculture
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.researchthemePlant Production and Health
cg.identifier.bibtexciteidSHENGE:2019
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAflatoxin
cg.iitasubjectFood Security
cg.iitasubjectGenetic Improvement
cg.iitasubjectMaize
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPlant Health
cg.journalFrontiers in Microbiology
cg.notesOpen Access Journal; Published online: 15 Nov 2019
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3389/fmicb.2019.02529
cg.iitaauthor.identifieradebowale akande: 0000-0002-6521-3272
cg.iitaauthor.identifierAlejandro Ortega-Beltran: 0000-0003-3747-8094
cg.iitaauthor.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.iitaauthor.identifierRanajit Bandyopadhyay: 0000-0003-2422-4298


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