• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Validation of diagnostic markers for streak virus disease resistance in maize

    Thumbnail
    View/Open
    Journal Article (1.056Mb)
    Date
    2021
    Author
    Sime, S.S.
    Menkir, A.
    Adetimirin, V.O.
    Gedil, M.
    Kumar, P.L.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Maize streak virus (MSV) is responsible for streak disease of maize and poses a serious threat to maize production in sub-Saharan Africa. Polygenic resistance to MSV has become an essential requirement in modern maize cultivars to mitigate yield losses. Many single nucleotide polymorphism (SNP) markers linked to putative MSV resistance loci have been identified for use in forward breeding. This study aimed to validate, using the high-throughput kompetitive allele specific PCR (KASP) assay, the diagnostic ability of the three SNP markers linked to the loci for the Msv1 resistance trait in 151 early generations inbred lines with diverse genetic backgrounds, together with nine MSV-resistant elite lines and a susceptible check (cv. Pool-16). The phenotypic responses were determined by MSV inoculation using viruliferous leafhoppers (Cicadulina triangular) under screenhouse conditions. Based on an established MSV disease rating system, the maize lines were categorized into resistant, moderately resistant, susceptible, and highly susceptible. The three SNPs associated with MSV resistance were detected in 133 lines, which were categorized as resistant (54), moderately resistant (76), and susceptible (1). The 18 early generation lines without these SNPs were classified as moderately resistant (10), susceptible (5), and highly susceptible (3). This study confirms the strong association of SNPs with MSV resistance and their usefulness for forward breeding in maize while emphasizing the need to identify additional markers to screen lines for MSV resistance without any ambiguity.
    https://dx.doi.org/10.3390/agriculture11020130
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7086
    IITA Authors ORCID
    Abebe Menkirhttps://orcid.org/0000-0002-5907-9177
    Melaku Gedilhttps://orcid.org/0000-0002-6258-6014
    P. Lava Kumarhttps://orcid.org/0000-0003-4388-6510
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/agriculture11020130
    Research Themes
    Biotech and Plant Breeding; Plant Production and Health
    IITA Subjects
    Agronomy; Maize; Plant Breeding; Plant Health; Plant Production
    Agrovoc Terms
    Maize; Maize Streak Virus; Phenotypes; Genotypes; Breeding; Single Nucleotide Polymorphism
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Agriculture
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository