• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Cocoyam (Colocasia esculenta) provides an effective monoxenic culture media for Radopholus similis

    Thumbnail
    View/Open
    Journal Article (388.5Kb)
    Date
    2021
    Author
    Oduori, C.A.
    Atandi, J.
    Kisaakye, J.
    Coyne, D.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    The burrowing nematode, Radopholus similis, is the most economically important nematode parasite of bananas, in addition to parasitising a range of other crops (Coyne & Kidane, 2018). Weakened, infected roots result in poor plant development and they are less able to provide anchorage to banana plants, which can lead to toppling of the entire plant. To enable research activities, such as resistance screening and efficacy studies, a readily available supply of inoculum is required for timely use in experiments. O’Bannon & Taylor (1968) developed the sterile carrot disc technique for the monoxenic culture of R. similis to support such routine research activities. This technique has since been adapted and employed for the monoxenic culture of a few root-lesion nematodes (see Kagoda et al., 2010; Santos et al., 2012). However, not all species are well adapted to this technique with some requiring months to develop, such as Pratylenchus sudanensis (Mudiope et al., 2004), and others proving unsuccessful, such as Helicotylenchus multicinctus (Speijer & De Waele, 1997). In addition, differences between carrot cultivars can lead to variations in nematode multiplication, with the most suitable cultivars not always available. Cultivars with a high water content tend to be more prone to greater levels of contamination, compared with carrot cultivars with a lower water content, which are denser (Coyne et al., 2014). In order to identify an alternative to carrot, cocoyam (Colocasia esculenta) was assessed for its suitability for culturing R. similis.
    https://dx.doi.org/10.1163/15685411-bja10088
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7130
    IITA Authors ORCID
    Daniel Coynehttps://orcid.org/0000-0002-2030-6328
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1163/15685411-bja10088
    Research Themes
    Natural Resource Management
    IITA Subjects
    Agronomy; Crop Systems; Plant Breeding; Plant Diseases; Plant Production
    Agrovoc Terms
    Taro; Nematodes; Rearing Systems; Reproduction; Screening; Lesions
    Regions
    Africa; East Africa
    Countries
    Kenya
    Hubs
    Eastern Africa Hub
    Journals
    Nematology
    Collections
    • Journal and Journal Articles4842
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository