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    Transgenic expression of dsRNA targeting the Pentalonia nigronervosa acetylcholinesterase gene in banana and plantain reduces aphid populations

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    Journal Article (5.255Mb)
    Date
    2021
    Author
    Jekayinoluwa, T.
    Tripathi, J.
    Dugdale, B.
    Obiero, G.
    Muge, E.
    Tripathi, L.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The banana aphid, Pentalonia nigronervosa, is the sole insect vector of banana bunchy top virus (BBTV), the causal agent of banana bunchy top disease. The aphid acquires and transmits BBTV while feeding on infected banana plants. RNA interference (RNAi) enables the generation of pest and disease-resistant crops; however, its effectiveness relies on the identification of pivotal gene sequences to target and silence. Acetylcholinesterase (AChE) is an essential enzyme responsible for the hydrolytic metabolism of the neurotransmitter acetylcholine in animals. In this study, the AChE gene of the banana aphid was targeted for silencing by RNAi through transgenic expression of AChE dsRNA in banana and plantain plants. The efficacy of dsRNA was first assessed using an artificial feeding assay. In vitro aphid feeding on a diet containing 7.5% sucrose, and sulfate complexes of trace metals supported aphid growth and reproduction. When AChE dsRNA was included in the diet, a dose of 500 ng/μL was lethal to the aphids. Transgenic banana cv. Cavendish Williams and plantain cvs. Gonja Manjaya and Orishele expressing AChE dsRNA were regenerated and assessed for transgene integration and copy number. When aphids were maintained on elite transgenic events, there was a 67.8%, 46.7%, and 75.6% reduction in aphid populations growing on Cavendish Williams, Gonja Manjaya, and Orishele cultivars, respectively, compared to those raised on nontransgenic control plants. These results suggest that RNAi targeting an essential aphid gene could be a useful means of reducing both aphid infestation and potentially the spread of the disease they transmit.
    https://dx.doi.org/10.3390/plants10040613
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7161
    IITA Authors ORCID
    Dr Jaindra Nath Tripathi, Ph.D.https://orcid.org/0000-0002-6366-917X
    Leena Tripathihttps://orcid.org/0000-0001-5723-4981
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/plants10040613
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Banana; Genetic Improvement; Plant Breeding; Plant Diseases; Plant Production; Plantain
    Agrovoc Terms
    Bananas; Plantains; Rna; Acetylcholinesterase; Aphidoidea; Sugars; Diet; Plant Diseases
    Regions
    Africa; East Africa
    Countries
    Kenya
    Hubs
    Eastern Africa Hub
    Journals
    Plants
    Collections
    • Journal and Journal Articles4836
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