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Insights in the global genetics and gut microbiome of black soldier fly, Hermetia illucens: implications for animal feed safety control

dc.contributor.authorKhamis, F.M.
dc.contributor.authorOmbura, F.L.
dc.contributor.authorAkutse, K.S.
dc.contributor.authorSubramanian, S.
dc.contributor.authorMohamed, S.A.
dc.contributor.authorFiaboe, K.K.
dc.contributor.authorSaijuntha, W.
dc.contributor.authorvan Loon, J.J.
dc.contributor.authorDicke, M.
dc.contributor.authorDubois, T.
dc.contributor.authorEkesi, S.
dc.contributor.authorTanga, C.M.
dc.date.accessioned2022-01-18T12:50:40Z
dc.date.available2022-01-18T12:50:40Z
dc.date.issued2020-07-07
dc.identifier.citationKhamis, F.M., Ombura, F.L., Akutse, K.S., Subramanian, S., Mohamed, S.A., Fiaboe, K.K., ... & Tanga, C.M.. (2020). Insights in the global genetics and gut microbiome of black soldier fly, Hermetia illucens: implications for animal feed safety control. Frontiers in Microbiology, 11:1538, 1-15.
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7314
dc.description.abstractThe utilization of the black soldier fly (BSF) Hermetia illucens L. for recycling organic waste into high-quality protein and fat biomass for animal feeds has gained momentum worldwide. However, information on the genetic diversity and environmental implications on safety of the larvae is limited. This study delineates genetic variability and unravels gut microbiome complex of wild-collected and domesticated BSF populations from six continents using mitochondrial COI gene and 16S metagenomics. All sequences generated from the study linked to H. illucens accessions KM967419.1, FJ794355.1, FJ794361.1, FJ794367.1, KC192965.1, and KY817115.1 from GenBank. Phylogenetic analyses of the sequences generated from the study and rooted by GenBank accessions of Hermetia albitarsis Fabricius and Hermetia sexmaculata Macquart separated all samples into three branches, with H. illucens and H. sexmaculata being closely related. Genetic distances between H. illucens samples from the study and GenBank accessions of H. illucens ranged between 0.0091 and 0.0407 while H. sexmaculata and H. albitarsis samples clearly separated from all H. illucens by distances of 0.1745 and 0.1903, respectively. Genetic distance matrix was used to generate a principal coordinate plot that further confirmed the phylogenetic clustering. Haplotype network map demonstrated that Australia, United States 1 (Rhode Island), United States 2 (Colorado), Kenya, and China shared a haplotype, while Uganda shared a haplotype with GenBank accession KC192965 BSF from United States. All other samples analyzed had individual haplotypes. Out of 481,695 reads analyzed from 16S metagenomics, four bacterial families (Enterobactereaceae, Dysgonomonadaceae, Wohlfahrtiimonadaceae, and Enterococcaceae) were most abundant in the BSF samples. Alpha-diversity, as assessed by Shannon index, showed that the Kenyan and Thailand populations had the highest and lowest microbe diversity, respectively; while microbial diversity assessed through Bray Curtis distance showed United States 3 (Maysville) and Netherlands populations to be the most dissimilar. Our findings on genetic diversity revealed slight phylogeographic variation between BSF populations across the globe. The 16S data depicted larval gut bacterial families with economically important genera that might pose health risks to both animals and humans. This study recommends pre-treatment of feedstocks and postharvest measures of the harvested BSF larvae to minimize risk of pathogen contamination along the insect-based feed value chain.
dc.description.sponsorshipNetherlands Organization for Scientific Research
dc.description.sponsorshipCanadian International Development Research Centre
dc.description.sponsorshipAustralian Centre for International Agricultural Research
dc.description.sponsorshipRockefeller Foundation
dc.description.sponsorshipUnited Kingdom Aid
dc.description.sponsorshipSwedish International Development Cooperation Agency
dc.description.sponsorshipSwiss Agency for Development and Cooperation
dc.description.sponsorshipFederal Ministry for Economic Cooperation and Development, Germany
dc.description.sponsorshipFederal Democratic Republic of Ethiopia
dc.description.sponsorshipKenyan Government
dc.format.extent1-15
dc.language.isoen
dc.subjectGenetic Diversity
dc.subjectHermetia Illucens
dc.subjectAnimal Feeds
dc.subjectControl Systems
dc.titleInsights in the global genetics and gut microbiome of black soldier fly, Hermetia illucens: implications for animal feed safety control
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Centre of Insect Physiology and Ecology
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMahasarakham University
cg.contributor.affiliationWageningen University and Research Centre
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.regionEurope
cg.coverage.regionNorth America
cg.coverage.regionSouthern Africa
cg.coverage.regionWest Africa
cg.coverage.countryAustralia
cg.coverage.countryChina
cg.coverage.countryCosta Rica
cg.coverage.countryGhana
cg.coverage.countryKenya
cg.coverage.countryNigeria
cg.coverage.countrySouth Africa
cg.coverage.countryThailand
cg.coverage.countryThe Netherlands
cg.coverage.countryUganda
cg.coverage.hubCentral Africa Hub
cg.researchthemeNatural Resource Management
cg.identifier.bibtexciteidKHAMIS:2020
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectNatural Resource Management
cg.iitasubjectPests of Plants
cg.iitasubjectPlant Genetic Resources
cg.journalFrontiers in Microbiology
cg.notesOpen Access Journal; Published online: 07 July 2020
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3389/fmicb.2020.01538
cg.futureupdate.requiredNo
cg.identifier.issue1538
cg.identifier.volume11


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