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    Preliminary assessment of the association between DArT-SEQ SNP and some nutritional traits in African yam bean

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    Journal Article (723.4Kb)
    Date
    2021
    Author
    Oluwole, O.O.
    Olomitutu, O.E.
    Paliwal, R.
    Oyatomi, O.
    Abberton, M.
    Obembe, O.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    African yam bean (AYB) is an underutilized tropical legume rich in protein and starch content. This study's aim was to use Diversity Arrays Technology (DArT) sequencing to study the ge-nome-wide association studies (GWAS) of nutritional traits. The study was carried out on 137 accessions in IITA, Ibadan. Seeds were harvested at maturity and analysed for protein, oil, and starch using Kjeltec, Soxtec, and Phenol-sulfuric acid assay method. Mixed Linear Model (MLM) was used for GWAS analysis by employing the TASSEL software. AYB protein content ranged from 17.13-27.15%, while the oil and starch content ranged from 0.74-2.27% and 60.58-69.84%, respectively. A total of 3.6K SNP was generated using a high-throughput DArTseq genotype-by-sequencing SNP approach in the AYB population. These generated SNPs were filtered for quality control, and the remaining useful 2.48K SNPs were used for the preliminary GWAS. We identified a promising source of genetic material for important grain nutritional traits that could be used in AYB breeding research. Identification of QTL/gene of protein, oil, and starch will contribute to our understanding of the genetic basis of natural variation in AYB grain nutritional traits.
    https://dx.doi.org/10.26538/tjnpr/v1i4.5
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7339
    IITA Authors ORCID
    Michael Abbertonhttps://orcid.org/0000-0003-2555-9591
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.26538/tjnpr/v1i4.5
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Crop Systems; Farming Systems; Food Security; Grain Legumes; Plant Breeding; Plant Production; Yam
    Agrovoc Terms
    African Yam Beans; Single Nucleotide Polymorphism; Legumes; Nutrition; Quantitative Trait Loci
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Tropical Journal of Natural Product Research
    Collections
    • Journal and Journal Articles4835
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