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    Continuous mapping identifies Loci associated with weevil resistance [Cosmopolites sordidus (Germar)] in a triploid banana population

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    Journal Article (1.802Mb)
    Date
    2021
    Author
    Uwimana, B.
    Mwanje, G.
    Batte, M.
    Akech, V.
    Shah, T.
    Vuylsteke, M.
    Swennen, R.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The first step towards marker-assisted selection is linking the phenotypes to molecular markers through quantitative trait loci (QTL) analysis. While the process is straightforward with self-pollinating diploid species, QTL analysis in polyploids requires unconventional methods. In this study, we have identified markers associated with weevil Cosmopolites sordidus (Germar) resistance in banana using 138 triploid (2n = 3x) hybrids derived from a cross between a tetraploid ‘Monyet’ (2n = 4x) and a diploid ‘Kokopo’ (2n = 2x) banana genotypes. The population was genotyped by DArTSeq, resulting in 18,009 polymorphic SNPs between the two parents. Marker–trait association was carried out by continuous mapping where the adjusted trait means for corm peripheral damage (PD) and total cross-section damage (TXD), both on the logit scale, were regressed on the marker allele frequencies. Forty-four SNPs were identified that were associated with corm peripheral damage on the chromosomes 5, 6 and 8 with 41 of them located on chromosome 6 and segregating in ‘Kokopo’. Eleven SNPs associated with corm total cross-section damage were identified on chromosome 6 and segregating in ‘Monyet’. The additive effect of replacing one reference allele with the alternative allele was determined at each marker position. The peripheral damage QTL was confirmed using conventional QTL linkage analysis in the simplex markers segregating in ‘Kokopo’ (AAAA × RA). We also identified 43 putative genes in the vicinity of the markers significantly associated with the two traits. The identified loci associated with resistance to weevil damage will be used in the efforts of developing molecular tools for marker-assisted breeding in banana.
    https://dx.doi.org/10.3389/fpls.2021.753241
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7347
    IITA Authors ORCID
    Brigitte Uwimanahttps://orcid.org/0000-0001-7460-9001
    Michael Battehttps://orcid.org/0000-0002-6793-2967
    Trushar Shahhttps://orcid.org/0000-0002-0091-7981
    Rony Swennenhttps://orcid.org/0000-0002-5258-9043
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3389/fpls.2021.753241
    Research Themes
    Biometrics; Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Banana; Biometrics; Food Security; Plant Breeding; Plant Production
    Agrovoc Terms
    Bananas; Weevils; Mapping; Cosmopolites Sordidus; Musa; Polyploidy; Quantitative Trait Loci; East Africa
    Regions
    Africa; East Africa
    Countries
    Uganda
    Hubs
    Eastern Africa Hub
    Journals
    Frontiers in Plant Science
    Collections
    • Journal and Journal Articles4835
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